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Literature summary for 2.3.1.87 extracted from

  • Okazaki, M.; Higuchi, K.; Hanawa, Y.; Shiraiwa, Y.; Ezura, H.
    Cloning and characterization of a Chlamydomonas reinhardtii cDNA arylalkylamine N-acetyltransferase and its use in the genetic engineering of melatonin content in the Micro-Tom tomato (2009), J. Pineal Res., 46, 373-382.
    View publication on PubMed
Search for more literature for 2.3.1.87

Cloned(Commentary)

Cloned (Comment) Organism
AANAT, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, expression in Micro-Tom, a model cultivar of Solanum lycopersicum, transgenic Micro-Tom exhibits higher melatonin content compared with wild type. Expression of N-terminally GST-tagged AANAT in Escherichia coli strain BL21 Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + serotonin Chlamydomonas reinhardtii rate-limiting in melatonin, i.e. N-acetyl-5-methoxytryptamine synthesis CoA + N-acetylserotonin
-
 ?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
 cDNA-coded arylalkylamine N-acetyltransferase
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information lacks cAMP-dependent protein kinase phosphorylation sites in the N- and C-terminal regions Chlamydomonas reinhardtii

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally GST-tagged AANAT from Escherichia coli strain BL21 by glutathione affinity chromatography Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + serotonin
-
 Chlamydomonas reinhardtii CoA + N-acetylserotonin
-
 ?
acetyl-CoA + serotonin rate-limiting in melatonin, i.e. N-acetyl-5-methoxytryptamine synthesis Chlamydomonas reinhardtii CoA + N-acetylserotonin
-
 ?

Synonyms

Synonyms Comment Organism
AANAT
-
 Chlamydomonas reinhardtii
arylalkylamine N-acetyltransferase
-
 Chlamydomonas reinhardtii

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the rate-limiting step in melatonin synthesis Chlamydomonas reinhardtii