Cloned (Comment) | Organism |
---|---|
baculovirus infection of Tn5 insect cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA-tryptamine | IC50: 0.00041 mM, GST-AANAT fusion protein | Homo sapiens | |
acetyl-CoA-tryptamine | IC50: 0.00062 mM, GST-AANAT fusion protein | Ovis aries | |
acetyl-CoA-tryptamine | IC50: 0.0008 mM, GST-AANAT fusion protein | Rattus norvegicus | |
bromoacetyltryptamine | IC50: 0.00143 mM, GST-AANAT fusion protein | Homo sapiens | |
bromoacetyltryptamine | IC50: 0.00128 mM, GST-AANAT fusion protein | Ovis aries | |
bromoacetyltryptamine | IC50: 0.0051 mM, GST-AANAT fusion protein | Rattus norvegicus | |
S 20251 | IC50: 0.002 mM, GST-AANAT fusion protein | Homo sapiens | |
S 20251 | IC50: 0.0013 mM, GST-AANAT fusion protein | Ovis aries | |
S 20251 | IC50: 0.0024 mM, GST-AANAT fusion protein | Rattus norvegicus | |
S 23823 | IC50: 0.005 mM, GST-AANAT fusion protein | Homo sapiens | |
S 23823 | IC50: 0.031 mM, GST-AANAT fusion protein | Rattus norvegicus | |
S 27244 | IC50: 0.00072 mM, GST-AANAT fusion protein | Homo sapiens | |
S 27244 | IC50: 0.00026 mM, GST-AANAT fusion protein | Ovis aries | |
S 27244 | IC50: 0.00036 mM, GST-AANAT fusion protein | Rattus norvegicus | |
S 27481 | IC50: 0.00018 mM, GST-AANAT fusion protein | Homo sapiens | |
S 27481 | IC50: 0.0004 mM, GST-AANAT fusion protein | Ovis aries | |
S 27481 | IC50: 0.0004 mM, GST-AANAT fusion protein | Rattus norvegicus | |
S 28036 | IC50: 0.0056 mM,GST-AANAT fusion protein | Homo sapiens | |
S 28036 | IC50: 0.00073 mM, GST-AANAT fusion protein | Ovis aries | |
S 28036 | IC50: 0.0017 mM, GST-AANAT fusion protein | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
2-Phenylethylamine | pH 6.8, 37°C | Homo sapiens | |
0.265 | - |
acetyl-CoA | pH 6.8, 37°C | Ovis aries | |
0.34 | - |
2-(2,3-dichlorophenyl)-ethylamine | pH 6.8, 37°C | Homo sapiens | |
0.47 | - |
2,5-dimethoxyphenylethylamine | pH 6.8, 37°C | Ovis aries | |
0.49 | - |
2-methoxyphenylethylamine | pH 6.8, 37°C | Ovis aries | |
0.53 | - |
acetyl-CoA | pH 6.8, 37°C | Homo sapiens | |
0.62 | - |
2-(3-chlorophenyl)-ethylamine | pH 6.8, 37°C | Homo sapiens | |
0.64 | - |
serotonin | pH 6.8, 37°C | Ovis aries | |
0.65 | - |
acetyl-CoA | pH 6.8, 37°C | Rattus norvegicus | |
0.75 | - |
2-(4-bromophenyl)-ethylamine | pH 6.8, 37°C | Homo sapiens | |
0.9 | - |
2-(2-chlorophenyl)-ethylamine | pH 6.8, 37°C | Ovis aries | |
0.98 | - |
2-(p-fluorophenyl)-ethylamine | pH 6.8, 37°C | Homo sapiens | |
1.1 | - |
2-(3-fluorophenyl)-ethylamine | pH 6.8, 37°C | Homo sapiens | |
1.17 | - |
2-(p-tolyl)-ethylamine | pH 6.8, 37°C | Homo sapiens | |
1.28 | - |
2-(4-fluorophenyl)-ethylamine | pH 6.8, 37°C | Ovis aries | |
1.3 | - |
2-methoxyphenylethylamine | pH 6.8, 37°C | Homo sapiens | |
1.31 | - |
2-(3-chlorophenyl)-ethylamine | pH 6.8, 37°C | Ovis aries | |
1.35 | - |
serotonin | pH 6.8, 37°C | Homo sapiens | |
1.37 | - |
2-fluorophenylethylamine | pH 6.8, 37°C | Ovis aries | |
1.37 | - |
2-fluorophenylethylamine | pH 6.8, 37°C | Homo sapiens | |
1.405 | - |
2-(p-nitrophenyl)-ethylamine | pH 6.8, 37°C | Ovis aries | |
1.55 | - |
2-(4-bromophenyl)-ethylamine | pH 6.8, 37°C | Ovis aries | |
1.58 | - |
2-(p-tolyl)-ethylamine | pH 6.8, 37°C | Ovis aries | |
1.65 | - |
2-(3-fluorophenyl)-ethylamine | pH 6.8, 37°C | Ovis aries | |
1.7 | - |
serotonin | pH 6.8, 37°C | Rattus norvegicus | |
1.76 | - |
2-(3,4-dihydroxyphenyl)-ethylamine | pH 6.8, 37°C | Ovis aries | |
1.8 | - |
2-Phenylethylamine | pH 6.8, 37°C | Ovis aries | |
1.815 | - |
3-methoxytyramine | pH 6.8, 37°C | Ovis aries | |
1.9 | - |
6-Hydroxydopamine | pH 6.8, 37°C | Homo sapiens | |
1.91 | - |
2-(p-fluorophenyl)-ethylamine | pH 6.8, 37°C | Ovis aries | |
2.07 | - |
3-methoxy-2-phenylethylamine | pH 6.8, 37°C | Ovis aries | |
2.2 | - |
2-(4-fluorophenyl)-ethylamine | pH 6.8, 37°C | Homo sapiens | |
2.33 | - |
2-(4-chlorophenyl)ethylamine | pH 6.8, 37°C | Ovis aries | |
2.74 | - |
2-(2-chlorophenyl)-ethylamine | pH 6.8, 37°C | Homo sapiens | |
3.46 | - |
2-(2,3-dichlorophenyl)-ethylamine | pH 6.8, 37°C | Ovis aries | |
9.5 | - |
2-Phenylethylamine | pH 6.8, 37°C | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q16613 | - |
- |
Ovis aries | - |
- |
- |
Rattus norvegicus | Q64666 | - |
- |
Purification (Comment) | Organism |
---|---|
denaturation/renaturation process in which the enzyme is immobilized on an affinity chromatography column | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.17 | - |
- |
Ovis aries |
10.57 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 2,5-dimethoxyphenylethylamine | 24% of the activity with 2-phenylethylamine | Homo sapiens | CoA + ? | - |
? | |
acetyl-CoA + 2,5-dimethoxyphenylethylamine | 68% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-2,5-dimethoxyphenylethylamine | - |
? | |
acetyl-CoA + 2-(2,3-dichlorophenyl)-ethylamine | 78% of the activity with 2-phenylethylamine | Homo sapiens | CoA + N-acetyl-2-(2,3-dichlorophenyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(2,3-dichlorophenyl)-ethylamine | 84% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-2-(2,3-dichlorophenyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(2-chlorophenyl)-ethylamine | 102% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-2-(2-chlorophenyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(2-chlorophenyl)-ethylamine | 130% of the activity with 2-phenylethylamine | Homo sapiens | CoA + N-acetyl-2-(2-chlorophenyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(3,4-dihydroxyphenyl)-ethylamine | 64% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-2-(3,4-dihydroxyphenyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(3,4-dihydroxyphenyl)-ethylamine | 7% of the activity with 2-phenylethylamine | Homo sapiens | CoA + N-acetyl-2-(3,4-dihydroxyphenyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(3,4-dimethoxyphenyl)-ethylamine | 15% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-2-(3,4-dimethoxyphenyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(3,4-dimethoxyphenyl)-ethylamine | 4.4% of the activity with 2-phenylethylamine | Homo sapiens | CoA + N-acetyl-2-(3,4-dimethoxyphenyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(3-chlorophenyl)-ethylamine | 110% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-2-(3-chlorophenyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(3-chlorophenyl)-ethylamine | 67% of the activity with 2-phenylethylamine | Homo sapiens | CoA + N-acetyl-2-(3-chlorophenyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(3-fluorophenyl)-ethylamine | 65% of the activity with 2-phenylethylamine | Homo sapiens | CoA + N-acetyl-2-(3-fluorophenyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(3-fluorophenyl)-ethylamine | 93% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-2-(3-fluorophenyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(4-bromophenyl)-ethylamine | 48% of the activity with 2-phenylethylamine | Homo sapiens | CoA + N-acetyl-2-(4-bromophenyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(4-bromophenyl)-ethylamine | 87% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-2-(4-bromophenyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(4-chlorophenyl)ethylamine | 68% of the activity with 2-phenylethylamine | Homo sapiens | CoA + N-acetyl-2-(4-chlorophenyl)ethylamine | - |
? | |
acetyl-CoA + 2-(4-chlorophenyl)ethylamine | 91% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-2-(4-chlorophenyl)ethylamine | - |
? | |
acetyl-CoA + 2-(4-fluorophenyl)-ethylamine | 116% of the activity with 2-phenylethylamine | Homo sapiens | CoA + N-acetyl-2-(4-fluorophenyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(4-fluorophenyl)-ethylamine | 60% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-2-(4-fluorophenyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(p-fluorophenyl)-ethylamine | 56% of the activity with 2-phenylethylamine | Homo sapiens | CoA + N-acetyl-2-(p-fluorophenyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(p-fluorophenyl)-ethylamine | as active as with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-2-(p-fluorophenyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(p-nitrophenyl)-ethylamine | 53% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-2-(p-nitrophenyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(p-nitrophenyl)-ethylamine | 9% of the activity with 2-phenylethylamine | Homo sapiens | CoA + N-acetyl-2-(p-nitrophenyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(p-tolyl)-ethylamine | 60% of the activity with 2-phenylethylamine | Homo sapiens | CoA + N-acetyl-2-(p-tolyl)-ethylamine | - |
? | |
acetyl-CoA + 2-(p-tolyl)-ethylamine | 87% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-2-(p-tolyl)-ethylamine | - |
? | |
acetyl-CoA + 2-fluorophenylethylamine | 69% of the activity with 2-phenylethylamine | Homo sapiens | CoA + ? | - |
? | |
acetyl-CoA + 2-fluorophenylethylamine | 52% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-2-fluorophenylethylamine | - |
? | |
acetyl-CoA + 2-methoxyphenylethylamine | 52% of the activity with 2-phenylethylamine | Homo sapiens | CoA + N-acetyl-2-methoxyphenylethylamine | - |
? | |
acetyl-CoA + 2-methoxyphenylethylamine | 97% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-2-methoxyphenylethylamine | - |
? | |
acetyl-CoA + 2-phenylethylamine | - |
Ovis aries | CoA + N-acetyl-2-phenylethylamine | - |
? | |
acetyl-CoA + 2-phenylethylamine | - |
Rattus norvegicus | CoA + N-acetyl-2-phenylethylamine | - |
? | |
acetyl-CoA + 2-phenylethylamine | - |
Homo sapiens | CoA + N-acetyl-2-phenylethylamine | - |
? | |
acetyl-CoA + 3-hydroxy-4-methoxyphenethylamine | 1% of the activity with 2-phenylethylamine | Homo sapiens | CoA + ? | - |
? | |
acetyl-CoA + 3-hydroxy-4-methoxyphenethylamine | 7.2% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-3-hydroxy-4-methoxyphenethylamine | - |
? | |
acetyl-CoA + 3-hydroxyphenethylamine | 24% of the activity with 2-phenylethylamine | Homo sapiens | CoA + ? | - |
? | |
acetyl-CoA + 3-hydroxyphenethylamine | 36% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-3-hydroxyphenethylamine | - |
? | |
acetyl-CoA + 3-methoxy-2-phenylethylamine | 23% of the activity with 2-phenylethylamine | Homo sapiens | CoA + ? | - |
? | |
acetyl-CoA + 3-methoxy-2-phenylethylamine | 55% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-3-methoxy-2-phenylethylamine | - |
? | |
acetyl-CoA + 3-methoxytyramine | 17% of the activity with 2-phenylethylamine | Homo sapiens | CoA + ? | - |
? | |
acetyl-CoA + 3-methoxytyramine | 73% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-3-methoxytyramine | - |
? | |
acetyl-CoA + 4-(2-aminoethyl)-benzenesulfonamide | 1% of the activity with 2-phenylethylamine | Ovis aries | CoA + ? | - |
? | |
acetyl-CoA + 4-(2-aminoethyl)-benzenesulfonyl fluoride | 7.4% of the activity with 2-phenylethylamine | Ovis aries | CoA + ? | - |
? | |
acetyl-CoA + 4-(2-aminoethyl)-benzenesulfonyl fluoride | 8% of the activity with 2-phenylethylamine | Homo sapiens | CoA + ? | - |
? | |
acetyl-CoA + 4-methoxyphenylethylamine | 10% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-4-methoxyphenylethylamine | - |
? | |
acetyl-CoA + 4-methoxyphenylethylamine | 11% of the activity with 2-phenylethylamine | Homo sapiens | CoA + N-acetyl-4-methoxyphenylethylamine | - |
? | |
acetyl-CoA + 5-hydroxydopamine | 4.4% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-5-hydroxydopamine | - |
? | |
acetyl-CoA + 6-hydroxydopamine | 51% of the activity with 2-phenylethylamine | Homo sapiens | CoA + ? | - |
? | |
acetyl-CoA + 6-hydroxydopamine | 1.5% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyl-6-hydroxydopamine | - |
? | |
acetyl-CoA + serotonin | - |
Ovis aries | CoA + N-acetylserotonin | - |
? | |
acetyl-CoA + serotonin | - |
Rattus norvegicus | CoA + N-acetylserotonin | - |
? | |
acetyl-CoA + serotonin | - |
Homo sapiens | CoA + N-acetylserotonin | - |
? | |
acetyl-CoA + tyramine | 10% of the activity with 2-phenylethylamine | Ovis aries | CoA + N-acetyltyramine | - |
? | |
acetyl-CoA + tyramine | 8% of the activity with 2-phenylethylamine | Homo sapiens | CoA + ? | - |
? | |
additional information | no activity with 2-(4-aminophenyl)-ethylamine, 3,4-(dibenzyloxy)phenethylamine or 2-(p-chlorophenoxy)-2-methylpropionic acid | Ovis aries | ? | - |
? | |
additional information | no activity with 2-(4-aminophenyl)-ethylamine, 3,4-(dibenzyloxy)phenethylamine, 5-hydroxydopamine, 4-(2-aminoethyl)-benzenesulfonamide or 2-(p-chlorophenoxy)-2-methylpropionic acid | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hAANAT | - |
Homo sapiens |
oAANAT | - |
Ovis aries |
oAANAT | - |
Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00018 | - |
IC50: 0.00018 mM, GST-AANAT fusion protein | Homo sapiens | S 27481 | |
0.00026 | - |
IC50: 0.00026 mM, GST-AANAT fusion protein | Ovis aries | S 27244 | |
0.00036 | - |
IC50: 0.00036 mM, GST-AANAT fusion protein | Rattus norvegicus | S 27244 | |
0.0004 | - |
IC50: 0.0004 mM, GST-AANAT fusion protein | Ovis aries | S 27481 | |
0.0004 | - |
IC50: 0.0004 mM, GST-AANAT fusion protein | Rattus norvegicus | S 27481 | |
0.00041 | - |
IC50: 0.00041 mM, GST-AANAT fusion protein | Homo sapiens | acetyl-CoA-tryptamine | |
0.00062 | - |
IC50: 0.00062 mM, GST-AANAT fusion protein | Ovis aries | acetyl-CoA-tryptamine | |
0.00072 | - |
IC50: 0.00072 mM, GST-AANAT fusion protein | Homo sapiens | S 27244 | |
0.00073 | - |
IC50: 0.00073 mM, GST-AANAT fusion protein | Ovis aries | S 28036 | |
0.0008 | - |
IC50: 0.0008 mM, GST-AANAT fusion protein | Rattus norvegicus | acetyl-CoA-tryptamine | |
0.00128 | - |
IC50: 0.00128 mM, GST-AANAT fusion protein | Ovis aries | bromoacetyltryptamine | |
0.0013 | - |
IC50: 0.0013 mM, GST-AANAT fusion protein | Ovis aries | S 20251 | |
0.00143 | - |
IC50: 0.00143 mM, GST-AANAT fusion protein | Homo sapiens | bromoacetyltryptamine | |
0.0017 | - |
IC50: 0.0017 mM, GST-AANAT fusion protein | Rattus norvegicus | S 28036 | |
0.002 | - |
IC50: 0.002 mM, GST-AANAT fusion protein | Homo sapiens | S 20251 | |
0.0024 | - |
IC50: 0.0024 mM, GST-AANAT fusion protein | Rattus norvegicus | S 20251 | |
0.005 | - |
IC50: 0.005 mM, GST-AANAT fusion protein | Homo sapiens | S 23823 | |
0.0051 | - |
IC50: 0.0051 mM, GST-AANAT fusion protein | Rattus norvegicus | bromoacetyltryptamine | |
0.0056 | - |
IC50: 0.0056 mM,GST-AANAT fusion protein | Homo sapiens | S 28036 | |
0.031 | - |
IC50: 0.031 mM, GST-AANAT fusion protein | Rattus norvegicus | S 23823 |