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Literature summary for 2.3.1.82 extracted from
- Mechanistic characterization of the bifunctional aminoglycoside-modifying enzyme AAC(3)-Ib/AAC(6)-Ib from Pseudomonas aeruginosa (2007), Biochemistry, 46, 5270-5282.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| both acetyltransferase domains of the bifunctional enzyme AAC(3)-Ib/AAC(6')-Ib' are cloned | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| butyryl-CoA | - |
Pseudomonas aeruginosa |  |
| CoASH | - |
Pseudomonas aeruginosa | |
| N6'-acetyl kanamycin A | - |
Pseudomonas aeruginosa | |
| paromomycin | - |
Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0003 | - |
isepamicin | reaction with AAC (6?)-Ib' domain | Pseudomonas aeruginosa | |
| 0.001 | - |
kanamycin A | reaction with AAC (6?)-Ib' domain | Pseudomonas aeruginosa | |
| 0.001 | - |
amikacin | reaction with AAC (6?)-Ib' domain | Pseudomonas aeruginosa | |
| 0.001 | - |
netilmicin | reaction with AAC (6?)-Ib' domain | Pseudomonas aeruginosa | |
| 0.0012 | - |
neomycin | reaction with AAC (6?)-Ib' domain | Pseudomonas aeruginosa | |
| 0.003 | - |
dibekacin | reaction with AAC (6?)-Ib' domain | Pseudomonas aeruginosa | |
| 0.0083 | - |
gentamicin | reaction with AAC (6?)-Ib' domain | Pseudomonas aeruginosa | |
| 0.009 | - |
acetyl-CoA | reaction with AAC (6?)-Ib' domain | Pseudomonas aeruginosa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| both acetyltransferase domains of the bifunctional enzyme AAC(3)-Ib/AAC(6')-Ib' are cloned and purified | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + amikacin | - |
Pseudomonas aeruginosa | CoA + N6'-acetylamikacin | - |
? | |
| acetyl-CoA + dibekacin | - |
Pseudomonas aeruginosa | CoA + N6'-acetyldibekacin | - |
? | |
| acetyl-CoA + gentamicin | - |
Pseudomonas aeruginosa | CoA + N6'-acetylgentamicin | - |
? | |
| acetyl-CoA + isepamicin | - |
Pseudomonas aeruginosa | CoA + N6'-acetylisepamicin | - |
? | |
| acetyl-CoA + kanamycin A | - |
Pseudomonas aeruginosa | CoA + N6'-acetylkanamycin A | - |
? | |
| acetyl-CoA + neomycin | - |
Pseudomonas aeruginosa | CoA + N6'-acetylneomycin | - |
? | |
| acetyl-CoA + netilmicin | - |
Pseudomonas aeruginosa | CoA + N6'-acetylnetilmicin | - |
? | |
| additional information | no activity with fortimicin A. Bifunctional antibiotic-resistance enzyme. Both domains are acetyltransferases. The AAC(3)-Ib domain appears to be highly specific to fortimicin A and gentamicin as substrates, while the AAC(6')-b'?domain exhibits a broad substrate spectrum | Pseudomonas aeruginosa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AAC(3)-Ib/AAC(6')-Ib' | bifunctional antibiotic-resistance enzyme. Both domains are acetyltransferases. The AAC(3)-Ib domain appears to be highly specific to fortimicin A and gentamicin as substrates, while the AAC(6')-b' domain (EC 2.3.1.82) exhibits a broad substrate spectrum | Pseudomonas aeruginosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
acetyl-CoA | reaction with AAC (6')-Ib' domain | Pseudomonas aeruginosa | |
| 1.3 | - |
netilmicin | reaction with AAC (6')-Ib' domain | Pseudomonas aeruginosa | |
| 1.3 | - |
dibekacin | reaction with AAC (6')-Ib' domain | Pseudomonas aeruginosa | |
| 2 | - |
gentamicin | reaction with AAC (6')-Ib' domain | Pseudomonas aeruginosa | |
| 2.4 | - |
kanamycin A | reaction with AAC (6')-Ib' domain | Pseudomonas aeruginosa | |
| 2.4 | - |
isepamicin | reaction with AAC (6')-Ib' domain | Pseudomonas aeruginosa | |
| 3.5 | - |
amikacin | reaction with AAC (6')-Ib' domain | Pseudomonas aeruginosa | |
| 6 | - |
neomycin | reaction with AAC (6')-Ib' domain | Pseudomonas aeruginosa | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0012 | - |
paromomycin | versus kanamycin A, K(is) | Pseudomonas aeruginosa | |
| 0.02 | - |
CoASH | versus kanamycin A. K(is) | Pseudomonas aeruginosa | |
| 0.03 | - |
CoASH | versus acetyl-CoA, K(is) | Pseudomonas aeruginosa | |
| 0.04 | - |
paromomycin | versus acetyl-CoA, K(ii) | Pseudomonas aeruginosa | |
| 0.04 | - |
6'-N-acetylated kanamycin A | versus acetyl-CoA. K(is) | Pseudomonas aeruginosa | |
| 0.05 | - |
6'-N-acetylated kanamycin A | versus kanamycin A. K(is) | Pseudomonas aeruginosa | |
| 0.11 | - |
butyryl-CoA | versus acetyl-CoA, K(is) | Pseudomonas aeruginosa | |
| 0.15 | - |
butyryl-CoA | versus kanamycin A, K(is) | Pseudomonas aeruginosa | |
| 0.2 | - |
CoASH | versus kanamycin A. K(ii) | Pseudomonas aeruginosa | |
| 0.38 | - |
6'-N-acetylated kanamycin A | versus acetyl-CoA. K(ii) | Pseudomonas aeruginosa | |
| 0.55 | - |
6'-N-acetylated kanamycin A | versus kanamycin A. K(ii) | Pseudomonas aeruginosa | |
| 0.57 | - |
butyryl-CoA | versus kanamycin A, K(ii) | Pseudomonas aeruginosa | |
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