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Literature summary for 2.3.1.82 extracted from
- Kinetic studies of aminoglycoside acetyltransferase and phosphotransferase from Staphylococcus aureus RPAL. Relationship between the two activities (1983), Eur. J. Biochem., 133, 515-521.
General Stability
| General Stability | Organism |
|---|---|
| GTP, CoA, gentamicin C, neamine protect against thermal inactivation | Staphylococcus aureus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6'-N-acetylneamine | product inhibition | Staphylococcus aureus |  |
| AMP | - |
Staphylococcus aureus | |
| CoA | product inhibition | Staphylococcus aureus | |
| gentamicin C1 | competitive inhibitor of neamine, non-competitive inhibitor of acetyl-CoA | Staphylococcus aureus | |
| additional information | no inhibition by GTP | Staphylococcus aureus | |
| paromomycin | inhibits reaction with neamine | Staphylococcus aureus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
sucrose density gradient centrifugation | Staphylococcus aureus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Staphylococcus aureus | involved in resistance to antibiotics | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
harbouring plasmid RPAL | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| unseparable from 2-O-aminoglycoside phosphotransferase by affinity chromatography or sucrose density gradient ultracentrifugation | Staphylococcus aureus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acetyl-CoA + kanamycin-B = CoA + N6'-acetylkanamycin-B | mechanism | Staphylococcus aureus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.304 | - |
- |
Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + gentamicin C1a | - |
Staphylococcus aureus | CoA + N6'-acetylgentamicin C1a | - |
? | |
| acetyl-CoA + kanamycin B | - |
Staphylococcus aureus | CoA + N6'-acetylkanamycin B | - |
? | |
| acetyl-CoA + neamine | - |
Staphylococcus aureus | CoA + 6'-N-acetylneamine | - |
? | |
| acetyl-CoA + neamine | - |
Staphylococcus aureus | CoA + N6'-acetylneamine | - |
? | |
| acetyl-CoA + tobramycin | - |
Staphylococcus aureus | CoA + N6'-acetyltobramycin | - |
? | |
| additional information | involved in resistance to antibiotics | Staphylococcus aureus | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
GTP, CoA, gentamicin C, neamine protect against thermal inactivation | Staphylococcus aureus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0036 | - |
CoA | pH 6.9, 37°C | Staphylococcus aureus | |
| 4 | - |
gentamicin C1 | pH 6.9, 37°C, inhibition of the reaction with neamine | Staphylococcus aureus | |
| 6 | - |
AMP | pH 6.9, 37°C, inhibition of the reaction with neamine | Staphylococcus aureus | |
| 7.7 | - |
paromomycin | pH 6.9, 37°C, inhibition of the reaction with neamine | Staphylococcus aureus | |
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