Cloned (Comment) | Organism |
---|---|
- |
Methanosarcina thermophila |
Protein Variants | Comment | Organism |
---|---|---|
C159A | Km similar to wild-type enzyme | Methanosarcina thermophila |
C159A/C277A/C312A/C325A | Km similar to wild-type enzyme | Methanosarcina thermophila |
C159S | Km similar to wild-type enzyme | Methanosarcina thermophila |
C277A | Km similar to wild-type enzyme | Methanosarcina thermophila |
C277A/C312A/C325A | Km similar to wild-type enzyme | Methanosarcina thermophila |
C312A | Km similar to wild-type enzyme | Methanosarcina thermophila |
C325A | Km similar to wild-type enzyme | Methanosarcina thermophila |
R133Q | increased Km for CoA, decreased Km for acetyl phosphate | Methanosarcina thermophila |
R287Q | decreased Km for CoA | Methanosarcina thermophila |
R28Q | increased Km | Methanosarcina thermophila |
R310Q | decreased Km for CoA | Methanosarcina thermophila |
R87Q | increased Km for CoA, decreased Km for acetyl phosphate | Methanosarcina thermophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | - |
Methanosarcina thermophila | |
Phenylglyoxal | 33% inhibition after preincubation with phenylglyoxal | Methanosarcina thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
CoA | R287Q mutant | Methanosarcina thermophila | |
0.037 | - |
CoA | R310Q mutant | Methanosarcina thermophila | |
0.043 | - |
acetyl phosphate | R87Q mutant | Methanosarcina thermophila | |
0.071 | - |
CoA | C159S mutant | Methanosarcina thermophila | |
0.073 | - |
acetyl phosphate | R133Q mutant | Methanosarcina thermophila | |
0.073 | - |
CoA | C159A/C277A/C312A/C325A mutant | Methanosarcina thermophila | |
0.078 | - |
CoA | C325A mutant | Methanosarcina thermophila | |
0.086 | - |
CoA | C277A/C312A/C325A mutant | Methanosarcina thermophila | |
0.089 | - |
CoA | wild-type after expression in E. coli | Methanosarcina thermophila | |
0.09 | - |
CoA | wild tpye | Methanosarcina thermophila | |
0.092 | - |
CoA | C312A mutant | Methanosarcina thermophila | |
0.093 | - |
CoA | C159A mutant | Methanosarcina thermophila | |
0.093 | - |
CoA | C277A mutant | Methanosarcina thermophila | |
0.166 | - |
acetyl phosphate | R287Q mutant | Methanosarcina thermophila | |
0.17 | - |
acetyl phosphate | wild tpye | Methanosarcina thermophila | |
0.18 | - |
acetyl phosphate | wild-type after expression in E. coli | Methanosarcina thermophila | |
0.187 | - |
acetyl phosphate | C277A mutant | Methanosarcina thermophila | |
0.191 | - |
acetyl phosphate | C159S mutant | Methanosarcina thermophila | |
0.198 | - |
acetyl phosphate | C312A mutant | Methanosarcina thermophila | |
0.206 | - |
acetyl phosphate | C159A mutant | Methanosarcina thermophila | |
0.222 | - |
acetyl phosphate | C159A/C277A/C312A/C325A mutant | Methanosarcina thermophila | |
0.23 | - |
acetyl phosphate | C325A mutant | Methanosarcina thermophila | |
0.253 | - |
CoA | R28Q mutant | Methanosarcina thermophila | |
0.254 | - |
acetyl phosphate | C277A/C312A/C325A mutant | Methanosarcina thermophila | |
0.747 | - |
CoA | R87Q mutant | Methanosarcina thermophila | |
0.775 | - |
acetyl phosphate | R310Q mutant | Methanosarcina thermophila | |
1.32 | - |
acetyl phosphate | R28Q mutant | Methanosarcina thermophila | |
1.694 | - |
CoA | R133Q mutant | Methanosarcina thermophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + phosphate | Methanosarcina thermophila | - |
CoA + acetyl phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + phosphate | - |
Methanosarcina thermophila | CoA + acetyl phosphate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.32 | - |
acetyl phosphate | C159A mutant | Methanosarcina thermophila | |
1.42 | - |
CoA | R310Q mutant | Methanosarcina thermophila | |
1.48 | - |
CoA | C159A mutant | Methanosarcina thermophila | |
1.63 | - |
acetyl phosphate | R310Q mutant | Methanosarcina thermophila | |
3.03 | - |
acetyl phosphate | C159A/C277A/C312A/C325A mutant | Methanosarcina thermophila | |
3.3 | - |
CoA | C159A/C277A/C312A/C325A mutant | Methanosarcina thermophila | |
4.93 | - |
acetyl phosphate | R87Q mutant | Methanosarcina thermophila | |
5.45 | - |
acetyl phosphate | mutant R287Q | Methanosarcina thermophila | |
5.95 | - |
CoA | mutant R287Q | Methanosarcina thermophila | |
12.5 | - |
acetyl phosphate | R133Q mutant | Methanosarcina thermophila | |
24.1 | - |
acetyl phosphate | mutant R28Q | Methanosarcina thermophila | |
24.6 | - |
CoA | R87Q mutant | Methanosarcina thermophila | |
26 | - |
CoA | mutant R28Q | Methanosarcina thermophila | |
40.6 | - |
acetyl phosphate | wild type | Methanosarcina thermophila | |
44.5 | - |
acetyl phosphate | C312A mutant | Methanosarcina thermophila | |
45.6 | - |
CoA | R133Q mutant | Methanosarcina thermophila | |
51.6 | - |
acetyl phosphate | C277A/C312A/C325A mutant | Methanosarcina thermophila | |
53.6 | - |
CoA | C277A/C312A/C325A mutant | Methanosarcina thermophila | |
53.8 | - |
CoA | wild type | Methanosarcina thermophila | |
55.4 | - |
CoA | C312A mutant | Methanosarcina thermophila | |
57.6 | - |
acetyl phosphate | C325A mutant | Methanosarcina thermophila | |
59.9 | - |
CoA | C325A mutant | Methanosarcina thermophila | |
66.3 | - |
acetyl phosphate | C159S mutant | Methanosarcina thermophila | |
68.5 | - |
acetyl phosphate | wild-type after expression in E. coli | Methanosarcina thermophila | |
73.5 | - |
CoA | C159S mutant | Methanosarcina thermophila | |
87.5 | - |
CoA | wild-type after expression in E. coli | Methanosarcina thermophila | |
94.9 | - |
acetyl phosphate | C277A mutant | Methanosarcina thermophila | |
100 | - |
CoA | C277A mutant | Methanosarcina thermophila |