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Literature summary for 2.3.1.8 extracted from
- The purification and properties of phosphotransacetylase (1952), J. Biol. Chem., 196, 527-534.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 1,3-dimercaptopropanol | activates at 0.01 M | Clostridium kluyveri |  |
| cysteine | activates at 0.01 M | Clostridium kluyveri | |
| hydrogen sulfide | activates at 0.01 M | Clostridium kluyveri | |
| thiolglycolic acid | activates at 0.01 M | Clostridium kluyveri | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| citrate buffer | 0.1 M, pH 8.0 | Clostridium kluyveri | |
| Diethylbarbiturate | potassium diethylbarbiturate buffer, 0.1 M, pH 8.0 | Clostridium kluyveri | |
| Li+ | 23% inhibition | Clostridium kluyveri | |
| Na+ | 85% inhibition | Clostridium kluyveri | |
| phosphate | inhibition of arsenolysis | Clostridium kluyveri | |
| potassium diphosphate | 0.1 M, pH 8.0 | Clostridium kluyveri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | activates | Clostridium kluyveri | |
| K+ | rate of arsenolysis increases with increasing potassium or ammonium salt concentration and reaches a maximum rate at 0.02 to 0.03 M salt concentration | Clostridium kluyveri | |
| NH4+ | rate of arsenolysis increases with increasing potassium or ammonium salt concentration and reaches a maximum rate at 0.02 to 0.03 M salt concentration | Clostridium kluyveri | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + phosphate | Clostridium kluyveri | - |
CoA + acetyl phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium kluyveri | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium kluyveri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 950 | - |
- |
Clostridium kluyveri |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.2 M phosphate or Tris-HCl, pH 8.0, several months | Clostridium kluyveri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + phosphate | - |
Clostridium kluyveri | CoA + acetyl phosphate | - |
r | |
| arsenate + acetyl-CoA | - |
Clostridium kluyveri | acetyl arsenate + CoA | - |
? | |
| additional information | arsenolysis | Clostridium kluyveri | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 8.2 | - |
Clostridium kluyveri |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8.6 | pH 6.8: about 25% of activity maximum, pH 8.6: about 90% of activity maximum | Clostridium kluyveri |
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