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Literature summary for 2.3.1.77 extracted from
- Partial purification and specificity of triacylglycerol: sterol acyltransferase from Sinapsis alba (1981), Phytochemistry, 20, 1799-1803.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| purification has to be carried out at 0-4°C | Sinapis alba |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | reagents containing free sulfhydryl groups e.g. DTT have no effect | Sinapis alba |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell membrane | - |
Sinapis alba | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | divalent metal ions have no stimulatory effect | Sinapis alba |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
two peaks, one more than 100000 Da, the other 18000 Da: the fraction with higher molecular weight may consist of the aggregated form of the enzyme of 18000 Da | Sinapis alba |
| 18000 | - |
gel filtration | Sinapis alba |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Sinapis alba | the acyltransferase in Sinapis alba roots exhibits group specificity for natural sterols, which are 3beta-hydroxysterols containing a planar ring system and differing only in number or position of double bonds or in the structure of side chain | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sinapis alba | - |
white mustard | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Sinapis alba |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | of seedling | Sinapis alba | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, solubilized enzyme preparation, 24 h, 10% loss of activity | Sinapis alba |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme has an absolute requirement for a beta-configuration of the hydroxyl group at C-3, rate of esterification is higher for sterols containing a planar ring system, triacylglycerols with unsaturated fatty acids are used more efficiently than fatty acids saturated with equal chain length, triacylglycerols with fatty acids C6-C22 can be used as acyl donors | Sinapis alba | ? | - |
? | |
| additional information | the acyltransferase in Sinapis alba roots exhibits group specificity for natural sterols, which are 3beta-hydroxysterols containing a planar ring system and differing only in number or position of double bonds or in the structure of side chain | Sinapis alba | ? | - |
? | |
| triacylglycerol + cholest-5-en-3beta-ol | triacylglycerol from olive oil | Sinapis alba | diacylglycerols + cholesteryl ester | - |
? | |
| trilinoleoylglycerol + cholest-5-en-3beta-ol | - |
Sinapis alba | dilinoleoylglycerol + cholesteryl linoleate | - |
? | |
| trioleoylglycerol + cholest-5-en-3beta-ol | - |
Sinapis alba | dioleoylglycerol + cholesteryl oleate | - |
? | |
| tripalmitoylglycerol + 24R-methylcholest-5-en-3beta-ol | i.e. campestero l | Sinapis alba | ? | - |
? | |
| tripalmitoylglycerol + 5alpha-androstan-3beta-ol | i.e. androstanol | Sinapis alba | ? | - |
? | |
| tripalmitoylglycerol + 5alpha-cholestan-3beta-ol | i.e. cholestanol, highest rate of esterification | Sinapis alba | ? | - |
? | |
| tripalmitoylglycerol + 5beta-cholestan-3beta-ol | i.e. coprostanol | Sinapis alba | ? | - |
? | |
| tripalmitoylglycerol + stigmast-5-en-3beta-ol | i.e. sitosterol | Sinapis alba | ? | - |
? | |
| tripalmitoylglycerol + stigmasta-5,22-dien-3beta-ol | i.e. stigmasterol | Sinapis alba | ? | - |
? | |
| tristearoylglycerol + cholest-5-en-3beta-ol | - |
Sinapis alba | distearoylglycerol + cholesteryl stearate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 52 | - |
- |
Sinapis alba |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | - |
assay at | Sinapis alba |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6.8 | - |
Sinapis alba |
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Release 2023.1 (January 2023)
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