Application | Comment | Organism |
---|---|---|
analysis | development of an enzymic assay by indirect measurement of fatty alcohol and fatty acyl-CoA esterification catalyzed by the WS/DGAT enzyme using the coupled reaction of 5,5'-dithiobis(2-nitrobenzoic acid) with free CoA liberated during the esterification reaction | Marinobacter nauticus |
analysis | development of an enzymic assay by indirect measurement of fatty alcohol and fatty acyl-CoA esterification catalyzed by the WS/DGAT enzyme using the coupled reaction of 5,5'-dithiobis(2-nitrobenzoic acid) with free CoA liberated during the esterification reaction | Acinetobacter baylyi |
analysis | development of an enzymic assay by indirect measurement of fatty alcohol and fatty acyl-CoA esterification catalyzed by the WS/DGAT enzyme using the coupled reaction of 5,5'-dithiobis(2-nitrobenzoic acid) with free CoA liberated during the esterification reaction | Rhodococcus jostii |
analysis | development of an enzymic assay by indirect measurement of fatty alcohol and fatty acyl-CoA esterification catalyzed by the WS/DGAT enzyme using the coupled reaction of 5,5'-dithiobis(2-nitrobenzoic acid) with free CoA liberated during the esterification reaction | Psychrobacter cryohalolentis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Marinobacter nauticus |
expression in Escherichia coli | Acinetobacter baylyi |
expression in Escherichia coli | Rhodococcus jostii |
expression in Escherichia coli | Psychrobacter cryohalolentis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baylyi | - |
- |
- |
Marinobacter nauticus | - |
- |
- |
Psychrobacter cryohalolentis | - |
- |
- |
Psychrobacter cryohalolentis K5 | - |
- |
- |
Rhodococcus jostii | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.08 | - |
substrate hexadecanol, pH 7.0, 22°C | Acinetobacter baylyi |
0.08 | - |
substrate hexadecanol, pH 7.0, 22°C | Psychrobacter cryohalolentis |
0.12 | - |
substrate hexadecanol, pH 7.0, 22°C | Rhodococcus jostii |
0.24 | - |
substrate hexadecanol, isoform Ma2, pH 7.0, 22°C | Marinobacter nauticus |
0.68 | - |
substrate hexadecanol, isoform Ma1, pH 7.0, 22°C | Marinobacter nauticus |
0.83 | - |
substrate dodecanol, pH 7.0, 22°C | Psychrobacter cryohalolentis |
0.84 | - |
substrate dodecanol, pH 7.0, 22°C | Acinetobacter baylyi |
3.37 | - |
substrate dodecanol, pH 7.0, 22°C | Rhodococcus jostii |
4.96 | - |
substrate dodecanol, isoform Ma2, pH 7.0, 22°C | Marinobacter nauticus |
37.3 | - |
substrate dodecanol, isoform Ma1, pH 7.0, 22°C | Marinobacter nauticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
decanol + palmitoyl-CoA | and undecanol, best substrates | Rhodococcus jostii | decanyl palmitate + CoA | - |
? | |
dodecanol + palmitoyl-CoA | - |
Rhodococcus jostii | dodecanyl palmitate + CoA | - |
? | |
dodecanol + palmitoyl-CoA | - |
Psychrobacter cryohalolentis | dodecanyl palmitate + CoA | - |
? | |
dodecanol + palmitoyl-CoA | best substrate | Acinetobacter baylyi | dodecanyl palmitate + CoA | - |
? | |
dodecanol + palmitoyl-CoA | and undecanol, best substrates for both isoforms Ma1 and Ma2 | Marinobacter nauticus | dodecanyl palmitate + CoA | - |
? | |
dodecanol + palmitoyl-CoA | - |
Psychrobacter cryohalolentis K5 | dodecanyl palmitate + CoA | - |
? | |
hexadecanol + palmitoyl-CoA | - |
Marinobacter nauticus | hexadecanyl palmitate + CoA | - |
? | |
hexadecanol + palmitoyl-CoA | - |
Acinetobacter baylyi | hexadecanyl palmitate + CoA | - |
? | |
hexadecanol + palmitoyl-CoA | - |
Psychrobacter cryohalolentis | hexadecanyl palmitate + CoA | - |
? | |
hexadecanol + palmitoyl-CoA | - |
Psychrobacter cryohalolentis K5 | hexadecanyl palmitate + CoA | - |
? | |
additional information | the order of addition, i.e.the substrate that the protein is incubated with during the first minute while the background absorbance is measured, has a significant impact on the rate of reaction. No substrate: hexanol | Marinobacter nauticus | ? | - |
? | |
additional information | the order of addition, i.e.the substrate that the protein is incubated with during the first minute while the background absorbance is measured, has a significant impact on the rate of reaction. No substrate: hexanol | Acinetobacter baylyi | ? | - |
? | |
additional information | the order of addition, i.e.the substrate that the protein is incubated with during the first minute while the background absorbance is measured, has a significant impact on the rate of reaction. No substrate: hexanol | Psychrobacter cryohalolentis | ? | - |
? | |
additional information | the order of addition, i.e.the substrate that the protein is incubated with during the first minute while the background absorbance is measured, has a significant impact on the rate of reaction. No substrate: hexanol | Psychrobacter cryohalolentis K5 | ? | - |
? | |
tetradecanol + palmitoyl-CoA | - |
Acinetobacter baylyi | tetradecanyl palmitate + CoA | - |
? | |
tetradecanol + palmitoyl-CoA | best substrate | Psychrobacter cryohalolentis | tetradecanyl palmitate + CoA | - |
? | |
tetradecanol + palmitoyl-CoA | best substrate | Psychrobacter cryohalolentis K5 | tetradecanyl palmitate + CoA | - |
? | |
undecanol + palmitoyl-CoA | - |
Acinetobacter baylyi | undecanyl palmitate + CoA | - |
? | |
undecanol + palmitoyl-CoA | and decanol, best substrates | Rhodococcus jostii | undecanyl palmitate + CoA | - |
? | |
undecanol + palmitoyl-CoA | and dodecanol, best substrates for both isoforms Ma1 and Ma2 | Marinobacter nauticus | undecanyl palmitate + CoA | - |
? | |
undecanol + palmitoyl-CoA | - |
Psychrobacter cryohalolentis | undecanyl palmitate + CoASH | - |
? | |
undecanol + palmitoyl-CoA | - |
Psychrobacter cryohalolentis K5 | undecanyl palmitate + CoASH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Rha1 | - |
Rhodococcus jostii |