Cloned (Comment) | Organism |
---|---|
screening, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 | Marinobacter nauticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of isozymes WS1 and WS2 | Marinobacter nauticus | |
0.044 | - |
hexadecanol | pH 7.4, 37°C, isozyme WS2 | Marinobacter nauticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + long-chain fatty alcohol | Marinobacter nauticus | - |
CoA + long-chain fatty ester | - |
? | |
additional information | Marinobacter nauticus | the enzyme is involved in synthesis of isoprenoid wax ester storage compounds, pathway, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Marinobacter nauticus | - |
DSM 8798, isozymes WS1, WS2, and WS3 | - |
Marinobacter nauticus | - |
DSM 8798 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acyl-CoA + a long-chain alcohol = CoA + a long-chain ester | the catalytic domain contains the highly conserved HHXXXDG motif | Marinobacter nauticus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | cells grown on phytol as the sole carbon source under limiting nitrogen and/or phosphorous conditions | Marinobacter nauticus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + long-chain fatty alcohol | - |
Marinobacter nauticus | CoA + long-chain fatty ester | - |
? | |
additional information | the enzyme is involved in synthesis of isoprenoid wax ester storage compounds, pathway, overview | Marinobacter nauticus | ? | - |
? | |
additional information | substrate profiles of isozymes WS1 and WS2, acyl-CoA specificity of isozyme WS2 with preference for palmitoyl-CoA and broad activity with alcohols of various chain lengths, bifunctional enzyme also catalyzing the reaction of EC 2.3.1.20 | Marinobacter nauticus | ? | - |
? | |
oleoyl-CoA + 1,2-dipalmitoyl-sn-glycerol | - |
Marinobacter nauticus | CoA + 1,2-dipalmitoyl-3-oleoyl-sn-glycerol | - |
? | |
palmitoyl-CoA + hexadecanol | - |
Marinobacter nauticus | CoA + hexadecyl palmitate | - |
? | |
palmitoyl-CoA + phytol | - |
Marinobacter nauticus | phytyl palmitate + CoA | - |
? | |
phytanoyl-CoA + phytol | - |
Marinobacter nauticus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
isoprenoid wax ester synthase | - |
Marinobacter nauticus |
More | cf. EC 2.3.1.20 | Marinobacter nauticus |
wax ester synthase | - |
Marinobacter nauticus |
wax ester synthase/diacylglycerol acyltransferase | - |
Marinobacter nauticus |
WS | - |
Marinobacter nauticus |
WS/DGAT | - |
Marinobacter nauticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Marinobacter nauticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4794 | - |
hexadecanol | pH 7.4, 37°C, isozyme WS2 | Marinobacter nauticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Marinobacter nauticus |