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Literature summary for 2.3.1.74 extracted from

  • Li, D.; Zheng, Q.; Zhang, H.
    A quantum mechanics study on the reaction mechanism of chalcone formation from p-coumaroyl-CoA and malonyl-CoA catalyzed by chalcone synthase (2009), Theoret. Chem. Accounts, 122, 157-166.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
using the crstal structure with PDB ID 1BQ6 for structure-function analysis, overview Medicago sativa

Protein Variants

Protein Variants Comment Organism
F215S the mutation does not significantly alter the Kd for CoA or acetyl-CoA binding, but dramatically alters the turnover rates for malonyl-CoA decarboxylation compared to the wild-type enzyme Medicago sativa
F215Y the mutation does not significantly alter the Kd for CoA or acetyl-CoA binding, but dramatically alters the turnover rates for malonyl-CoA decarboxylation compared to the wild-type enzyme Medicago sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 malonyl-CoA + 4-coumaroyl-CoA Medicago sativa
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4 CoA + naringenin chalcone + 3 CO2
-
?

Organism

Organism UniProt Comment Textmining
Medicago sativa P30074
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-

Reaction

Reaction Comment Organism Reaction ID
3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2 reaction mechanism analysis by quantum mechanics calculations, overview. In loading step, only a tetrahedral intermediate is located without transition state. His303 acts as a H31 donor, but not a hydrogen bond donor, to stabilize the intermediate formation. In decarboxylation step, the reaction proceeds via a transition state and is sensitive to the environment. In elongation step, a tetrahedral transition state is located, structure-function analysis, overview Medicago sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 malonyl-CoA + 4-coumaroyl-CoA
-
Medicago sativa 4 CoA + naringenin chalcone + 3 CO2
-
?

Synonyms

Synonyms Comment Organism
chalcone synthase
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Medicago sativa
CHS
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Medicago sativa