Cloned (Comment) | Organism |
---|---|
- |
Sinapis alba |
Protein Variants | Comment | Organism |
---|---|---|
C169S | no enzyme activity | Sinapis alba |
C169S/Q100E | 28% of wild-type activity | Sinapis alba |
C169S/Q100E/K180Q | 15% of wild-type activity | Sinapis alba |
K180Q | no enzyme activity | Sinapis alba |
Q100E | 14% of wild-type activity | Sinapis alba |
S158C | 90% of wild-type activity | Sinapis alba |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
4-coumaroyl-CoA | C169S/Q100E/K180Q triple mutant | Sinapis alba | |
0.0025 | - |
4-coumaroyl-CoA | wild-type enzyme | Sinapis alba |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinapis alba | - |
white mustard | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl CoA + 4-coumaroyl-CoA + NADH | - |
Sinapis alba | 6'-deoxychalcone + CoA + CO2 + NAD+ | the formation of this product requires an additional reductase | ? |