Any feedback?
Literature summary for 2.3.1.7 extracted from
- Substrate specificity of human carnitine acetyltransferase: Implications for fatty acid and branched-chain amino acid metabolism (2013), Biochim. Biophys. Acta, 1832, 773-779.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.078 | - |
acetyl-CoA | peroxisomal enzyme, at pH 7.4 and 37°C | Homo sapiens |  |
| 0.24 | - |
acetyl-CoA | mitochondrial enzyme, at pH 7.4 and 37°C | Homo sapiens | |
| 0.499 | - |
butyryl-CoA | mitochondrial enzyme, at pH 7.4 and 37°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
| peroxisome | - |
Homo sapiens | 5777 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisLink protein purification resin column chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + L-carnitine | - |
Homo sapiens | CoA + O-acetylcarnitine | - |
? | |
| butyryl-CoA + L-carnitine | - |
Homo sapiens | CoA + O-butyrylcarnitine | - |
? | |
| additional information | the enzyme shows some activity with acyl-CoAs of longer chain length including C12- and C14-CoA, but not with C16-CoA. Trans-2-enoyl-CoA intermediates such as trans-2-ene-C4-CoA (crotonyl-CoA, trans-2-C4:1-CoA), trans-2-ene-3-methyl-C4-CoA (3-methylcrotonyl-CoA), and trans-2-ene-2-methyl-C4-CoA are poor substrates for the enzyme and no activity is found with dicarboxylic acyl-CoA esters | Homo sapiens | ? | - |
? | |
| octanoyl-CoA + L-carnitine | - |
Homo sapiens | CoA + O-octanoylcarnitine | - |
? | |
| palmitoyl-CoA + L-carnitine | - |
Homo sapiens | CoA + O-palmitoylcarnitine | - |
? | |
| propionyl-CoA + L-carnitine | - |
Homo sapiens | CoA + O-propionylcarnitine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carnitine acetyltransferase | - |
Homo sapiens |
| CRAT | - |
Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
