Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli or Saccharomyces cerevisiae | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D356A/M564G | shows 6fold higher activity toward palmitoyl-CoA than that of the single mutant M564G and a new activity toward stearoyl-CoA. Mutations convert the enzyme into a pseudo carnitine palmitoyltransferase in terms of substrate specificity | Rattus norvegicus |
M564G | mutant enzyme shows higher activity with medium-chain acyl-CoAs than wild-type enzyme | Rattus norvegicus |
T465V/T467N/R518N | 3fold improved catalytic efficiency toward choline (Kcat/Km) compared with that of the wild-type enzyme | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0072 | - |
palmitoyl-CoA | mutant enzyme D356A/M564G | Rattus norvegicus | |
0.023 | - |
acetyl-CoA | mutant enzyme D356A/M564G | Rattus norvegicus | |
0.101 | - |
carnitine | wild-type enzyme | Rattus norvegicus | |
18.1 | - |
choline | mutant enzyme A106M/T465V/T467N/R518N | Rattus norvegicus | |
29 | - |
choline | mutant enzyme T465V/T467N/R518N | Rattus norvegicus | |
86.4 | - |
choline | wild-type enzyme | Rattus norvegicus | |
260 | - |
carnitine | mutant enzyme T465V/T467N/R518N | Rattus norvegicus | |
300 | - |
carnitine | above, mutant enzyme A106M/T465V/T467N/R518N | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + carnitine | - |
Rattus norvegicus | CoA + O-acetylcarnitine | - |
? | |
acetyl-CoA + choline | low activity with wild-type enzyme. Mutant enzymes T465V/T467N/R518N and A106M/T465V/T467N/R518N show improved catalytic efficiency toward choline (Kcat/Km) compared with that of the wild type enzyme | Rattus norvegicus | acetylcholine + CoA | - |
? | |
butyryl-CoA + carnitine | wild-type enzyme shows maximal activity towards butyryl-CoA | Rattus norvegicus | CoA + O-butyrylcarnitine | - |
? | |
decanoyl-CoA + carnitine | - |
Rattus norvegicus | CoA + O-decanoylcarnitine | - |
? | |
hexanoyl-CoA + carnitine | mutant enzymes M564G and D356A/M564G show maximal activity towards hexanoyl-CoA | Rattus norvegicus | CoA + O-hexanoylcarnitine | - |
? | |
octanoyl-CoA + carnitine | - |
Rattus norvegicus | CoA + O-octanoylcarnitine | - |
? | |
palmitoyl-CoA + carnitine | wild-type enzyme shows no activity, mutant enzymes M564G and D356A/M564G show activity | Rattus norvegicus | CoA + O-palmitoylcarnitine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.98 | - |
carnitine | above, mutant enzyme A106M/T465V/T467N/R518N | Rattus norvegicus | |
1.58 | - |
choline | wild-type enzyme | Rattus norvegicus | |
2.43 | - |
choline | mutant enzyme T465V/T467N/R518N | Rattus norvegicus | |
2.97 | - |
carnitine | mutant enzyme T465V/T467N/R518N | Rattus norvegicus | |
3.11 | - |
choline | mutant enzyme A106M/T465V/T467N/R518N | Rattus norvegicus | |
86.9 | - |
carnitine | wild-type enzyme | Rattus norvegicus |