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Literature summary for 2.3.1.7 extracted from

  • Claus, R.; Kaeppeli, O.; Fiechter, A.
    Purification of carnitine acetyltransferase from Candida tropicalis (1982), Anal. Biochem., 127, 376-379.
    View publication on PubMed
Search for more literature for 2.3.1.7

Application

Application Comment Organism
analysis specific activity is higher than in commercial preparations from other sources, enzyme prepared from yeast may be used for determination of carnitine in biological materials, this preparation is free of acetyl-CoA-deacetylase activity Candida tropicalis
analysis in medicine and biochemical practice, the enzyme isolated from breast muscle is used analytically Columba sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 acetyl-CoA
-
 Candida tropicalis
3.3
-
 DL-carnitine
-
 Candida tropicalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 SDS-PAGE Candida tropicalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + carnitine Candida tropicalis
-
 O-acetylcarnitine + CoASH
-
 r
acetyl-CoA + carnitine Columba sp.
-
 O-acetylcarnitine + CoASH
-
 r
acyl-CoA + carnitine Candida tropicalis
-
 acylcarnitine + CoASH
-
 r
acyl-CoA + carnitine Columba sp.
-
 acylcarnitine + CoASH
-
 r
acylcarnitine + CoASH Candida tropicalis
-
 acyl-CoA + carnitine
-
 r
acylcarnitine + CoASH Columba sp.
-
 acyl-CoA + carnitine
-
 r
O-acetylcarnitine + CoASH Candida tropicalis
-
 acetyl-CoA + carnitine
-
 r
O-acetylcarnitine + CoASH Columba sp.
-
 acetyl-CoA + carnitine
-
 r

Organism

Organism UniProt Comment Textmining
Candida tropicalis
-
 yeast
-
Candida tropicalis
-
 ATCC 32113
-
Columba sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Candida tropicalis

Source Tissue

Source Tissue Comment Organism Textmining
breast muscle
-
 Columba sp.
-
muscle
-
 Columba sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.021
-
-
 Candida tropicalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + carnitine
-
 Candida tropicalis O-acetylcarnitine + CoASH
-
 r
acetyl-CoA + carnitine
-
 Columba sp. O-acetylcarnitine + CoASH
-
 r
acetyl-CoA + L-(-)carnitine
-
 Candida tropicalis O-acetylcarnitine + CoASH
-
 r
acetyl-CoA + L-(-)carnitine
-
 Columba sp. O-acetylcarnitine + CoASH
-
 r
acyl-CoA + carnitine
-
 Candida tropicalis acylcarnitine + CoASH
-
 r
acyl-CoA + carnitine
-
 Columba sp. acylcarnitine + CoASH
-
 r
acylcarnitine + CoASH
-
 Candida tropicalis acyl-CoA + carnitine
-
 r
acylcarnitine + CoASH
-
 Columba sp. acyl-CoA + carnitine
-
 r
malonyl-CoA + L-(-)carnitine
-
 Candida tropicalis malonylcarnitine + CoASH
-
 r
malonylcarnitine + CoASH slight transfer of the acyl group to carnitine Candida tropicalis malonyl-CoA + L-(-)carnitine
-
 r
additional information
-
 Columba sp. ?
-
 ?
additional information crotonyl-CoA is no substrate Candida tropicalis ?
-
 ?
n-butyryl-CoA + carnitine
-
 Candida tropicalis n-butyrylcarnitine + CoASH
-
 r
n-butyrylcarnitine + CoASH
-
 Candida tropicalis n-butyryl-CoA + carnitine
-
 r
O-acetylcarnitine + CoASH
-
 Candida tropicalis acetyl-CoA + L-(-)carnitine
-
 r
O-acetylcarnitine + CoASH
-
 Columba sp. acetyl-CoA + L-(-)carnitine
-
 r
O-acetylcarnitine + CoASH
-
 Candida tropicalis acetyl-CoA + carnitine
-
 r
O-acetylcarnitine + CoASH
-
 Columba sp. acetyl-CoA + carnitine
-
 r
propionyl-CoA + L-(-)carnitine
-
 Candida tropicalis propionylcarnitine + CoASH
-
 r
propionylcarnitine + CoASH
-
 Candida tropicalis propionyl-CoA + L-(-)carnitine
-
 r