Literature summary for 2.3.1.67 extracted from

Harayama, T.; Shindou, H.; Ogasawara, R.; Suwabe, A.; Shimizu, T.
Identification of a novel noninflammatory biosynthetic pathway of platelet-activating factor (2008), J. Biol. Chem., 283, 11097-11106.

Search for more literature for 2.3.1.67

Cloned(Commentary)

Cloned (Comment)	Organism
for transfection of CHO-K1 cells	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
F174A	mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors	Mus musculus
I160A	mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors	Mus musculus
I162A	mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors	Mus musculus
I167A	mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors	Mus musculus
I170A	mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors	Mus musculus
L166A	mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors	Mus musculus
V173A	mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors	Mus musculus
V175A	mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors	Mus musculus
W163A	mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors	Mus musculus
Y169A	mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors	Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	-	Mus musculus
Fe2+	-	Mus musculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0824	-	acetyl-CoA	apparent KM	Mus musculus
0.1282	-	1-O-hexadecyl-sn-glycero-3-phosphocholine	apparent KM	Mus musculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Mus musculus	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	determined by SDS-PAGE and Western Blot analysis	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1-O-hexadecyl-sn-glycero-3-phosphocholine + acetyl-CoA	Mus musculus	lyso-PAF, lyso-platelet-activating factor	1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + CoA	PAF, platelet-activating factor	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	Q3TFD2	-	-

Purification (Commentary)

Purification (Comment)	Organism
LPCAT1 is partially purified from microsomes from mouse tissue using a DEAE-Sepharose column	Mus musculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Mus musculus	-
lung	-	Mus musculus	-
spleen	-	Mus musculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.000017	-	brain, Ca2+ independent lyso-PAF AT activity in the presence of EDTA, in the presence of Ca2+ 0.000104	Mus musculus
0.000104	-	spleen, Ca2+ independent lyso-PAF AT activity in the presence of EDTA, in the presence of Ca2+ 0.000470	Mus musculus
0.00033	-	lung, Ca2+ independent lyso-PAF AT activity in the presence of EDTA, in the presence of Ca2+ 0.000452	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-O-hexadecyl-sn-glycero-3-phosphocholine + acetyl-CoA	lyso-PAF, lyso-platelet-activating factor	Mus musculus	1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + CoA	PAF, platelet-activating factor	?
additional information	LPCAT1 recognizes a wide range of acyl-CoAs as substrates, ranging from acetyl-CoA to palmitoyl-CoA	Mus musculus	?	-	?

Synonyms

Synonyms	Comment	Organism
LPCAT1	Ca2+ independent lyso-PAF acetyltransferase	Mus musculus
lyso-PAF acetyltransferase	-	Mus musculus
lyso-PAF AT	-	Mus musculus
lysophosphatidylcholine acyltransferase 1	-	Mus musculus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	activity assay	Mus musculus

Cofactor

Cofactor	Comment	Organism	Structure
acetyl-CoA	-	Mus musculus

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Release 2023.1 (January 2023)