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Literature summary for 2.3.1.65 extracted from

  • Falany, C.N.; Fortinberry, H.; Leiter, E.H.; Barnes, S.
    Cloning, expression, and chromosomal localization of mouse liver bile acid CoA:amino acid N-acyltransferase (1997), J. Lipid Res., 38, 1139-1148.
    View publication on PubMed
Search for more literature for 2.3.1.65

Cloned(Commentary)

Cloned (Comment) Organism
cDNA encoding enzyme is cloned, sequenced and expressed in Escherichia coli DH5alpha, the open-reading frame codes for a 420 amino acid protein, Baat gene is mapped to mouse chromosome 4, single gene encodes enzyme Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by glycine Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9
-
 taurine recombinant enzyme, expressed in Escherichia coli Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 46525, calculated from the amino acid sequence, x * 45000, recombinant enzyme, SDS-PAGE Mus musculus
46525
-
 x * 46525, calculated from the amino acid sequence, x * 45000, recombinant enzyme, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
choloyl-CoA + taurine Mus musculus taurine-specific enzyme, gallbladder bile contains only taurine conjugates of bile acids, bile acid conjugates increases their detergent properties, prevents their precipitation in the acidic milieu of the upper small intestine, in intestines they are responsible for the solubilization and absorption of fats, vitamins and fat-soluble compounds CoA + taurocholate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification of recombinant enzyme, expressed in Escherichia coli DH5alpha Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Mus musculus
-
additional information not in heart, brain, spleen, lung, skeletal muscle, kidney or testis tissue Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
choloyl-CoA + taurine taurine-specific enzyme, no use of glycine or fluoro-beta-alanine as substrates Mus musculus CoA + taurocholate
-
 ?
choloyl-CoA + taurine taurine-specific enzyme, gallbladder bile contains only taurine conjugates of bile acids, bile acid conjugates increases their detergent properties, prevents their precipitation in the acidic milieu of the upper small intestine, in intestines they are responsible for the solubilization and absorption of fats, vitamins and fat-soluble compounds Mus musculus CoA + taurocholate
-
 ?
additional information not: glycine, fluoro-beta-alanine Mus musculus ?
-
 ?
additional information Cys-234 may be involved in the formation of a bile acid thioester at the active site Mus musculus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 46525, calculated from the amino acid sequence, x * 45000, recombinant enzyme, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
mBAT
-
 Mus musculus