General Stability | Organism |
---|---|
high ionic strength stabilizes | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cholate | - |
Bos taurus | |
Conjugated bile acids | efficient competitive inhibitors of cholyl-CoA binding | Bos taurus | |
glycocholate | competitive to cholyl-CoA, uncompetitive to glycine | Bos taurus | |
additional information | not inhibited by N-ethylmaleimide, iodoacetate | Bos taurus | |
NaCl | above 50 mM | Bos taurus | |
p-mercuribenzoate | reversible by dithioerythritol, substrates protect, serine and alanine protect | Bos taurus | |
taurocholate | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Bos taurus | |
0.0072 | - |
choloyl-CoA | - |
Bos taurus | |
6.7 | - |
taurine | - |
Bos taurus | |
8.8 | - |
glycine | - |
Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
choloyl-CoA + glycine | Bos taurus | taurine can replace glycine, involved in conjugation of bile acids | CoA + glycocholate | - |
? | |
choloyl-CoA + taurine | Bos taurus | taurine can replace glycine, involved in conjugation of bile acids | CoA + taurocholate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
choloyl-CoA + glycine = CoA + glycocholate | ping-pong mechanism | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
43 | 59 | glycine | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chenodeoxycholoyl-CoA + glycine | rate of conjugation in decreasing order: deoxycholyl-CoA, cholyl-CoA, lithocholyl-CoA, chenodeoxycholyl-CoA | Bos taurus | CoA + glycochenodeoxycholate | - |
? | |
choloyl-CoA + glycine | rate of conjugation in decreasing order: deoxycholyl-CoA, cholyl-CoA, lithocholyl-CoA, chenodeoxycholyl-CoA | Bos taurus | CoA + glycocholate | - |
ir | |
choloyl-CoA + glycine | taurine can replace glycine, involved in conjugation of bile acids | Bos taurus | CoA + glycocholate | - |
? | |
choloyl-CoA + taurine | - |
Bos taurus | CoA + taurocholate | - |
? | |
choloyl-CoA + taurine | taurine can replace glycine, involved in conjugation of bile acids | Bos taurus | CoA + taurocholate | - |
? | |
deoxycholoyl-CoA + glycine | rate of conjugation in decreasing order: deoxycholyl-CoA, cholyl-CoA, lithocholyl-CoA, chenodeoxycholyl-CoA | Bos taurus | CoA + glycodeoxycholate | - |
? | |
lithocholoyl-CoA + glycine | rate of conjugation in decreasing order: deoxycholyl-CoA, cholyl-CoA, lithocholyl-CoA, chenodeoxycholyl-CoA | Bos taurus | CoA + glycolithocholate | - |
? | |
additional information | not: L-serine, beta-alanine | Bos taurus | ? | - |
? | |
additional information | not as acyl-CoA donors: acetyl-CoA, phenylacetyl-CoA and benzoyl-CoA | Bos taurus | ? | - |
? | |
additional information | not: L-alpha-alanine, L-glutamine, L-ornithine | Bos taurus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | higher kcat for glycine than for taurine | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
glycocholate | - |
Bos taurus |