Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1-mercapto-2,3-propanediol | 1 mM is required for optimal activity | Hordeum vulgare | |
2-mercaptoethanol | 1 mM is required for optimal activity | Hordeum vulgare |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuSO4 | 10 mM, 85% inhibition | Hordeum vulgare | |
dithiothreitol | 30% inhibition | Hordeum vulgare | |
MnCl2 | 10 mM, 29% inhibition | Hordeum vulgare | |
ZnSO4 | 10 mM, 99% inhibition | Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
4-coumaroyl-CoA | isoenzyme 1 | Hordeum vulgare | |
0.0016 | - |
4-coumaroyl-CoA | isoenzyme 3 | Hordeum vulgare | |
0.0017 | - |
feruloyl-CoA | isoenzyme 3 | Hordeum vulgare | |
0.0021 | - |
4-coumaroyl-CoA | isoenzyme 2 | Hordeum vulgare | |
0.0021 | - |
4-coumaroyl-CoA | recombinant enzyme | Hordeum vulgare | |
0.0041 | - |
feruloyl-CoA | isoenzyme 2 | Hordeum vulgare | |
0.0043 | - |
caffeoyl-CoA | isoenzyme 2 | Hordeum vulgare | |
0.0043 | - |
caffeoyl-CoA | recombinant enzyme | Hordeum vulgare | |
0.0052 | - |
agmatine | isoenzyme 1 | Hordeum vulgare | |
0.0052 | - |
agmatine | isoenzyme 3 | Hordeum vulgare | |
0.0053 | - |
caffeoyl-CoA | isoenzyme 3 | Hordeum vulgare | |
0.0058 | - |
caffeoyl-CoA | isoenzyme 1 | Hordeum vulgare | |
0.0061 | - |
feruloyl-CoA | isoenzyme 1 | Hordeum vulgare | |
0.0065 | - |
agmatine | isoenzyme 2 | Hordeum vulgare | |
0.0066 | - |
feruloyl-CoA | recombinant enzyme | Hordeum vulgare | |
0.0081 | - |
agmatine | recombinant enzyme | Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
- |
Hordeum vulgare |
48000 | - |
1 * 48000, SDS-PAGE | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | A9ZPJ6 | isoenzymes 1-3 | - |
Purification (Comment) | Organism |
---|---|
Blue Sepharose, t-butyl-Sepharose, Resource Q, Superose 12 HR | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.78 | - |
- |
Hordeum vulgare |
11.34 | - |
recombinant enzyme | Hordeum vulgare |
Storage Stability | Organism |
---|---|
0°C, 100 mM Tris, pH 7.5, 1 mM EDTA, 10 mM 2-mercaptoethanol, 50 mM KCl | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + agmatine | - |
Hordeum vulgare | CoA + 4-coumaroylagmatine | - |
? | |
caffeoyl-CoA + agmatine | - |
Hordeum vulgare | CoA + caffeoylagmatine | - |
? | |
feruloyl-CoA + agmatine | - |
Hordeum vulgare | CoA + feruloyl agmatine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 48000, SDS-PAGE | Hordeum vulgare |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Hordeum vulgare |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | - |
Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Hordeum vulgare |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.9 | 8.1 | half-maximal activity at pH 6.9 and pH 8.1 | Hordeum vulgare |