KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00074 | - |
4-coumaroyl-CoA | - |
Hordeum vulgare | |
0.012 | - |
agmatine | - |
Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
gel filtration | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Biogel A, Sepharose 4B-agmatine | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | shoots, not seeds | Hordeum vulgare | - |
seedling | maximal activity 3-4 days after germination at 22°C in the dark | Hordeum vulgare | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.393 | - |
- |
Hordeum vulgare |
Storage Stability | Organism |
---|---|
-40°C, concentrated preparation at least 1 month, negligible loss of activity | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + agmatine | no activity with putrescine, spermidine, spermine, cadaverine, arginine, homoarginine | Hordeum vulgare | CoA + 4-coumaroylagmatine | - |
? | |
caffeoyl-CoA + agmatine | - |
Hordeum vulgare | CoA + caffeoylagmatine | - |
? | |
feruloyl-CoA + agmatine | - |
Hordeum vulgare | CoA + feruloyl agmatine | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Hordeum vulgare |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | approx. half-maximal activity at pH 7.0 and 8.0 | Hordeum vulgare |