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Literature summary for 2.3.1.6 extracted from

  • Cronin, C.N.
    Redesign of choline acetyltransferase specificity by protein engineering (1998), J. Biol. Chem., 273, 24465-24469.
    View publication on PubMed
Search for more literature for 2.3.1.6

Protein Variants

Protein Variants Comment Organism
N514R is described as ChAT-R, the introduction of an Arg at position 514 in rat enzyme is predicted to provide the ionic charge required to interact with, and neutralize, the carboxyl group of carnitine Rattus norvegicus
V459T/D460E/N461T is described as ChAT-TET Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00201
-
 acetyl-CoA cosubstrate carnitine Rattus norvegicus
0.00284
-
 acetyl-CoA enzyme mutant ChAT-R/TET, cosubstrate carnitine Rattus norvegicus
0.012
-
 acetyl-CoA enzyme mutant ChAT-R, cosubstrate carnitine Rattus norvegicus
0.0148
-
 acetyl-CoA enzyme mutant ChAT-TET, cosubstrate carnitine Rattus norvegicus
0.015
-
 acetyl-CoA cosubstrate choline Rattus norvegicus
0.0155
-
 acetyl-CoA enzyme mutant ChAT-R, cosubstrate choline Rattus norvegicus
0.0225
-
 acetyl-CoA enzyme mutant ChAT-TET, cosubstrate choline Rattus norvegicus
0.0516
-
 acetyl-CoA enzyme mutant ChAT-R/TET, cosubstrate acetyl-CoA Rattus norvegicus
0.192
-
 choline cosubstrate acetyl-CoA Rattus norvegicus
1.88
-
 choline enzyme mutant ChAT-R, cosubstrate acetyl-CoA Rattus norvegicus
4.8
-
 carnitine enzyme mutant ChAT-R/TET, cosubstrate acetyl-CoA Rattus norvegicus
5.79
-
 choline enzyme mutant ChAT-TET, cosubstrate acetyl-CoA Rattus norvegicus
100
-
 carnitine cosubstrate acetyl-CoA Rattus norvegicus
100
-
 carnitine enzyme mutant ChAT-R, cosubstrate acetyl-CoA Rattus norvegicus
100
-
 carnitine enzyme mutant ChAT-TET, cosubstrate acetyl-CoA Rattus norvegicus
128
-
 choline enzyme mutant ChAT-TET, cosubstrate acetyl-CoA Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + carnitine
-
 Rattus norvegicus acetylcarnitine + CoA
-
 ?
acetyl-CoA + choline
-
 Rattus norvegicus acetylcholine + CoA
-
 r