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Literature summary for 2.3.1.57 extracted from

  • Baek, J.H.; Liu, Y.V.; McDonald, K.R.; Wesley, J.B.; Zhang, H.; Semenza, G.L.
    Spermidine/spermine N(1)-acetyltransferase-1 binds to hypoxia-inducible factor-1alpha (HIF-1alpha) and RACK1 and promotes ubiquitination and degradation of HIF-1alpha (2007), J. Biol. Chem., 282, 33358-33366.
    View publication on PubMed
Search for more literature for 2.3.1.57

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-Gold(DE3)pLysS cells, transformed with pGEX expression vectors Homo sapiens

Protein Variants

Protein Variants Comment Organism
R101K site-directed mutagenesis of SSAT1 protein, ability to promote hypoxia-inducible factor-1 (HIF-1alpha) degradation reduced Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P21673 encodes SSAT1 protein
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Purification (Commentary)

Purification (Comment) Organism
gel filtration, recombinant protein Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
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 Homo sapiens
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HEK-293T cell
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 Homo sapiens
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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 SSAT1 inhibits hypoxia-inducible factor-1 (HIF-1alpha) through protein/protein interaction, effect of SSAT1 loss of function on HIF-1alpha protein levels and HIF-1 transcriptional activity, mutation of Arg101 impairs SSAT1-mediated HIF-1alpha degradation, SSAT1 promotes the ubiquitination of HIF-1alpha, involvement of SSAT1 and SSAT2 in alternative pathways for ubiquitination and degradation of HIF-1alpha, complementary roles in promoting O2-independent and O2-dependent degradation of HIF-1alpha Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information roles of SSAT proteins in oxygen homeostasis, SSAT1 binding to hypoxia-inducible factor-1 (HIF-1alpha) promotes its ubiquitination/degradation, in contrast to SSAT2, SSAT1 acts by stabilizing the interaction of HIF-1alpha with RACK1 Homo sapiens ?
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 ?

Synonyms

Synonyms Comment Organism
spermidine/spermine N1-acetyltransferase 1
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 Homo sapiens
SSAT1
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 Homo sapiens