Activating Compound | Comment | Organism | Structure |
---|---|---|---|
N1,N11-bis(ethyl)norspermine | polyamine analogue, about 300fold activation of wild-type enzyme, 4fold activation of mutant L156F, the compound highly stabilizes the wild-type enzyme preventing it from degradation, while the effect on degradation of the mutant enzyme is smaller | Cricetulus griseus |
Cloned (Comment) | Organism |
---|---|
expression of the L156F mutant enzyme in C55.7Res cells, in vitro transcription and translation of His-tagged wild-type and mutant L156F enzymes | Cricetulus griseus |
Protein Variants | Comment | Organism |
---|---|---|
L156F | mutant enzyme shows about 20fold reduced activity and over 70fold reduced activation by the polyamine analogue N1,N11-bis(ethyl)norspermine compared to the wild-type enzyme, expression of the mutant enzymes decreases cellular sensitivity to N1,N11-bis(ethyl)norspermine | Cricetulus griseus |
General Stability | Organism |
---|---|
N1,N11-bis(ethyl)norspermine completely stabilizes the wild-type enzyme, which is degraded within 90 min in absence of N1,N11-bis(ethyl)norspermine, half-life: 22 min, half-life in presence of N1,N11-bis(ethyl)norspermine: 144 h, while for the mutant L156F half-life is 105 min and 154 min, respectively | Cricetulus griseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
spermine | pH 7.8, 30°C, recombinant wild-type enzyme | Cricetulus griseus | |
0.037 | - |
spermine | pH 7.8, 30°C, recombinant mutant L156F enzyme | Cricetulus griseus | |
0.056 | - |
spermidine | pH 7.8, 30°C, recombinant wild-type enzyme | Cricetulus griseus | |
0.3 | - |
spermidine | pH 7.8, 30°C, recombinant mutant L156F enzyme | Cricetulus griseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cricetulus griseus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant L156F enzymes from in vitro transcription and translation | Cricetulus griseus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
CHO cell | - |
Cricetulus griseus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Cricetulus griseus |
1.5 | - |
purified recombinant mutant L156F | Cricetulus griseus |
26 | - |
purified recombinant wild-type enzyme | Cricetulus griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + spermidine | - |
Cricetulus griseus | CoA + N1-acetyl-spermidine | - |
? | |
acetyl-CoA + spermine | - |
Cricetulus griseus | CoA + N1-acetyl-spermine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
spermidine/spermine N1-acetyltransferase | - |
Cricetulus griseus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cricetulus griseus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.63 | - |
spermidine | pH 7.8, 30°C, recombinant mutant L156F enzyme | Cricetulus griseus | |
0.63 | - |
spermine | pH 7.8, 30°C, recombinant mutant L156F enzyme | Cricetulus griseus | |
5 | - |
spermine | pH 7.8, 30°C, recombinant wild-type enzyme | Cricetulus griseus | |
8.7 | - |
spermidine | pH 7.8, 30°C, recombinant wild-type enzyme | Cricetulus griseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Cricetulus griseus |