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Literature summary for 2.3.1.57 extracted from
- Spermidine N1-acetyltransferase (1983), Methods Enzymol., 94, 321-325.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme is induced by treatment with hepatotoxins, e.g. carbon tetrachloride | Rattus norvegicus | |
| additional information | enzyme is induced by growth hormone, thioacetamide, dialkylnitrosamines, folic acid and spermidine | Rattus norvegicus |
General Stability
| General Stability | Organism |
|---|---|
| sensitive to incubation in dilute solutions, bovine serum albumin, 0.5 mg/ml, stabilizes | Rattus norvegicus |
| unstable enzyme, spermidine stabilizes | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
2 * 60000, SDS-PAGE | Rattus norvegicus |
| 114000 | - |
- |
Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
female Sprague-Dawley rats | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 112000fold | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | carbon tetrachloride induced liver | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.102 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50 mM Tris-HCl buffer, pH 7.5, 10 mM spermidine, per week, 30% loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + 1,3-diaminopropane | at a low rate | Rattus norvegicus | CoA + N1-acetyl-1,3-diaminopropane | - |
? |  |
| acetyl-CoA + N1-acetylspermine | - |
Rattus norvegicus | CoA + N1,N12-diacetylspermine | - |
? | |
| acetyl-CoA + spermidine | - |
Rattus norvegicus | CoA + N1-acetylspermidine | product is exclusively N1-acetylspermidine | ? | |
| acetyl-CoA + spermine | - |
Rattus norvegicus | CoA + N1-acetylspermine | - |
? | |
| acetyl-CoA + sym-norspermidine | - |
Rattus norvegicus | CoA + N1-acetyl-sym-norspermidine | - |
? | |
| acetyl-CoA + sym-norspermine | - |
Rattus norvegicus | CoA + N1-acetyl-sym-norspermine | - |
? | |
| additional information | not: histone | Rattus norvegicus | ? | - |
? | |
| additional information | enzyme requires a substrate with the structure H2N(CH2)3NHR and acetylates the primary amino group | Rattus norvegicus | ? | - |
? | |
| additional information | not: putrescine | Rattus norvegicus | ? | - |
? | |
| additional information | not: sym-homospermidine | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 60000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
very sensitive to heat denaturation | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no known cofactors | Rattus norvegicus |
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