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Literature summary for 2.3.1.57 extracted from
- Purification and characterization of spermidine/spermine N1-acetyltransferase from rat liver (1982), Biochemistry, 21, 6152-6158.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme is induced by treatment with hepatotoxins, e.g. carbon tetrachloride | Rattus norvegicus |
General Stability
| General Stability | Organism |
|---|---|
| unstable enzyme, spermidine stabilizes | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3'-dephospho-CoA | less inhibition than by CoA | Rattus norvegicus |  |
| CoA | strong inhibitor | Rattus norvegicus | |
| CoA methyl disulfide | very strong inhibitor | Rattus norvegicus | |
| p-chloromercuribenzoate | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0015 | - |
acetyl-CoA | - |
Rattus norvegicus | |
| 0.008 | - |
sym-norspermidine | - |
Rattus norvegicus | |
| 0.03 | - |
N1-acetylspermine | - |
Rattus norvegicus | |
| 0.035 | - |
spermine | - |
Rattus norvegicus | |
| 0.1 | - |
1,5-Diaminopentane | - |
Rattus norvegicus | |
| 0.13 | - |
spermidine | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
2 * 60000, SDS-PAGE | Rattus norvegicus |
| 115000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + spermidine | Rattus norvegicus | spermidine and spermine may be physiological substrates, enzyme may play an important role in interconversion of polyamines | CoA + N1-acetylspermidine | - |
? | |
| acetyl-CoA + spermine | Rattus norvegicus | spermidine and spermine may be physiological substrates, enzyme may play an important role in interconversion of polyamines | CoA + N1-acetylspermine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 112000fold | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | carbon tetrachloride induced liver | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.102 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50 mM Tris-HCl buffer, pH 7.5, 10 mM spermidine, per week, 30% loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + 1,5-diaminopentane | - |
Rattus norvegicus | CoA + N1-acetyl-1,5-diaminopentane | - |
? | |
| acetyl-CoA + N1-acetylspermine | reaction in vitro, but not in vivo | Rattus norvegicus | CoA + N1,N12-diacetylspermine | - |
ir | |
| acetyl-CoA + spermidine | physiological substrate, higher rate than with spermine | Rattus norvegicus | CoA + N1-acetylspermidine | product is exclusively N1-acetylspermidine | ir | |
| acetyl-CoA + spermidine | spermidine and spermine may be physiological substrates, enzyme may play an important role in interconversion of polyamines | Rattus norvegicus | CoA + N1-acetylspermidine | - |
? | |
| acetyl-CoA + spermine | physiological substrate, lower rate than with spermidine | Rattus norvegicus | CoA + N1-acetylspermine | - |
ir | |
| acetyl-CoA + spermine | spermidine and spermine may be physiological substrates, enzyme may play an important role in interconversion of polyamines | Rattus norvegicus | CoA + N1-acetylspermine | - |
? | |
| acetyl-CoA + sym-norspermidine | high affinity for sym-norspermidine | Rattus norvegicus | CoA + N1-acetyl-sym-norspermidine | - |
? | |
| additional information | not: histone | Rattus norvegicus | ? | - |
? | |
| additional information | not: putrescine | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 60000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
half-life: 2.2 min, in 50 mM Tris-HCl, pH 7.5, 10 mM spermidine | Rattus norvegicus |
| 52 | - |
half-life: 0.5 min, in 50 mM Tris-HCl, pH 7.5, 10 mM spermidine | Rattus norvegicus |
| 60 | - |
0.5 min, 90% loss of activity, in 50 mM Tris-HCl, pH 7.5, 10 mM spermidine | Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
CoA | - |
Rattus norvegicus | |
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