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Literature summary for 2.3.1.51 extracted from
- SLC1 and SLC4 encode partially redundant acyl-coenzyme A 1-acylglycerol-3-phosphate O-acyltransferases of budding yeast (2007), J. Biol. Chem., 282, 30845-30855.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain HB101, transformed into Saccharomyces cerevisiae, strains, plasmids and sources of parent vectors listed | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Saccharomyces cerevisiae | 16020 | - |
| microsome | - |
Saccharomyces cerevisiae | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61180 | - |
partially purified Slc1p, SDS-PAGE, Western Blot | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | Saccharomyces cerevisiae | acyltranferase activity by Slc1p and Slc4p | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? |  |
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | Saccharomyces cerevisiae | Slc1p and Slc4p both active as acyltransferases, involved in fatty acid exchange of mature glycerophospholipids | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P33333 | 16 strains, genotypes listed | - |
| Saccharomyces cerevisiae | Q08548 | 16 strains, genotypes listed | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially purified, gel filtration | Saccharomyces cerevisiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Saccharomyces cerevisiae | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
deletion of Slc1p alone not lethal, microsomal enzyme activity not completely lost, evidence for additional enzyme activity, comparison between Slc1p with Slc4p, single mutants of Slc1p and Slc4p generated, double mutant of Slc1p and Slc4p lethal, same glycerophospholipid profiles but different lipid profiles between Slc1p and Slc4p, both enzymes able to use endogenous lysoglycerophospholipids as substrates, substrate specificity between Slc1p and Slc4p different or access to different lysoglycerophospholipid substrates because of a different subcellular location, Slc1p and Slc4p are both active as acyltransferases and are also involved in fatty acid exchange at the sn-2-position of mature glycerophospholipids | Saccharomyces cerevisiae |
| additional information | - |
microsomal enzyme activity not completely lost by deletion of Slc1p, evidence for additional enzyme activity, SLC4 gene encodes second enzmye indicated by lethality of double mutants of Slc1p and Slc4p, same glycerophospholipid profile but different lipid profiles between Slc1p and Slc4p, different substrate specificity between Slc1p and Slc4p or access to different lysoglycerophospholipid substrates because of a different subcellular location | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | acyltranferase activity by Slc1p and Slc4p | Saccharomyces cerevisiae | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | Slc1p and Slc4p both active as acyltransferases, involved in fatty acid exchange of mature glycerophospholipids | Saccharomyces cerevisiae | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | glycerophospholipid profile similar between Slc1p and Slc4p | Saccharomyces cerevisiae | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1-acylglycerol 3-phosphate O-acyltransferase | - |
Saccharomyces cerevisiae |
| Slc1p | - |
Saccharomyces cerevisiae |
| Slc4p | - |
Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
activity assay at | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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