Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | stimulation of the enzyme by incorporation of the enzyme into the artificial membrane vesicles or by stripping of CHAPS from the enzyme into mixed detergent-phospholipid micelles. There is a critical order in which assay components had to be combined to obtain maximum stimulation | Cocos nucifera |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | above 5 mM | Cocos nucifera | |
Ca2+ | 50% inhibition above 10 mM | Cocos nucifera | |
CHAPS | above 0.1% | Cocos nucifera | |
MgCl2 | 50% inhibition above 10 mM | Cocos nucifera | |
Mn2+ | 50% inhibition above 10 mM | Cocos nucifera | |
NaCl | above 200 mM | Cocos nucifera |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Cocos nucifera | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
estimated, high-speed supernatant, gel filtration | Cocos nucifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | Cocos nucifera | - |
CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
additional information | Cocos nucifera | the enzyme has medium chain length substrate specificity appropriate to the biosynthesis of coconut oil | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cocos nucifera | - |
coconut | - |
Renatured (Comment) | Organism |
---|---|
reassociation of the solubilized enzyme with soybean phospholipids | Cocos nucifera |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | immature | Cocos nucifera | - |
seed | - |
Cocos nucifera | - |
Storage Stability | Organism |
---|---|
4°C, high-speed supernatant preparation, 50% loss of activity, overnight | Cocos nucifera |
4°C, high-speed supernatant, reactivated with phospholipids, no loss of activity | Cocos nucifera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Cocos nucifera | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
additional information | acyl-CoAs containing 10:0, 12:0 and 14:0 acyl groups are the preferred acyl-donor substrates, acyl-ACPs are not utilized. There is a slight preference for 12:0-lysophosphatidic acid over 18:1-lysophosphatidic acid as acceptor substrate | Cocos nucifera | ? | - |
? | |
additional information | the enzyme has medium chain length substrate specificity appropriate to the biosynthesis of coconut oil | Cocos nucifera | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cocos nucifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Cocos nucifera |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | lowering the assay pH from 7.5 to 6.5 results in a loss of approximately 20% of the enzyme activity | Cocos nucifera |