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Literature summary for 2.3.1.51 extracted from

  • Davies, H.M.; Hawkins, D.J.; Nelsen, J.S.
    Lysophosphatidic acid acyltransferase from immature coconut endosperm having medium chain length substrate specificity (1995), Phytochemistry, 39, 989-996.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
additional information stimulation of the enzyme by incorporation of the enzyme into the artificial membrane vesicles or by stripping of CHAPS from the enzyme into mixed detergent-phospholipid micelles. There is a critical order in which assay components had to be combined to obtain maximum stimulation Cocos nucifera

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol above 5 mM Cocos nucifera
Ca2+ 50% inhibition above 10 mM Cocos nucifera
CHAPS above 0.1% Cocos nucifera
MgCl2 50% inhibition above 10 mM Cocos nucifera
Mn2+ 50% inhibition above 10 mM Cocos nucifera
NaCl above 200 mM Cocos nucifera

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Cocos nucifera 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
estimated, high-speed supernatant, gel filtration Cocos nucifera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate Cocos nucifera
-
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
?
additional information Cocos nucifera the enzyme has medium chain length substrate specificity appropriate to the biosynthesis of coconut oil ?
-
?

Organism

Organism UniProt Comment Textmining
Cocos nucifera
-
coconut
-

Renatured (Commentary)

Renatured (Comment) Organism
reassociation of the solubilized enzyme with soybean phospholipids Cocos nucifera

Source Tissue

Source Tissue Comment Organism Textmining
endosperm immature Cocos nucifera
-
seed
-
Cocos nucifera
-

Storage Stability

Storage Stability Organism
4°C, high-speed supernatant preparation, 50% loss of activity, overnight Cocos nucifera
4°C, high-speed supernatant, reactivated with phospholipids, no loss of activity Cocos nucifera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
Cocos nucifera CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
?
additional information acyl-CoAs containing 10:0, 12:0 and 14:0 acyl groups are the preferred acyl-donor substrates, acyl-ACPs are not utilized. There is a slight preference for 12:0-lysophosphatidic acid over 18:1-lysophosphatidic acid as acceptor substrate Cocos nucifera ?
-
?
additional information the enzyme has medium chain length substrate specificity appropriate to the biosynthesis of coconut oil Cocos nucifera ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Cocos nucifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Cocos nucifera

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 7.5 lowering the assay pH from 7.5 to 6.5 results in a loss of approximately 20% of the enzyme activity Cocos nucifera