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Literature summary for 2.3.1.50 extracted from
- Molecular characterization of membrane-associated soluble serine palmitoyltransferases from Sphingobacterium multivorum and Bdellovibrio stolpii (2007), J. Bacteriol., 189, 5749-5761.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Sphingobacterium multivorum |
| expressed in Escherichia coli | Sphingobacterium spiritivorum |
| expressed in Escherichia coli | Bacteriovorax stolpii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| palmitoyl CoA | enzyme shows remarkable substrate inhibition at palmitoyl-CoA concentrations higher than 0.1 nM | Bacteriovorax stolpii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
palmitoyl-CoA | - |
Sphingobacterium multivorum | |
| 0.39 | - |
palmitoyl-CoA | - |
Sphingobacterium spiritivorum | |
| 3.7 | - |
L-serine | - |
Bacteriovorax stolpii | |
| 4.8 | - |
L-serine | - |
Sphingobacterium multivorum | |
| 5 | - |
L-serine | - |
Sphingobacterium spiritivorum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| peripheral membrane protein | by immunoelectron microscopy it is shown that the protein is bound to the inner membrane of cells as peripheral membrane protein | Sphingobacterium multivorum | - |
- |
| peripheral membrane protein | by immunoelectron microscopy it is shown that the protein is bound to the inner membrane of cells as peripheral membrane protein | Sphingobacterium spiritivorum | - |
- |
| peripheral membrane protein | by immunoelectron microscopy it is shown that the protein is bound to the inner membrane of cells as peripheral membrane protein | Bacteriovorax stolpii | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
SDS-PAGE | Sphingobacterium multivorum |
| 45000 | - |
SDS-PAGE | Sphingobacterium spiritivorum |
| 50000 | - |
SDS-PAGE | Bacteriovorax stolpii |
| 90000 | - |
gel filtration | Sphingobacterium multivorum |
| 90000 | - |
gel filtration | Sphingobacterium spiritivorum |
| 90000 | - |
gel filtration | Bacteriovorax stolpii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacteriovorax stolpii | A7BFV8 | - |
- |
| Sphingobacterium multivorum | A7BFV6 | - |
- |
| Sphingobacterium spiritivorum | A7BFV7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified as a water-soluble active homodimer | Sphingobacterium spiritivorum |
| purified as a water-soluble active homodimer | Bacteriovorax stolpii |
| purified as water-soluble active homodimers | Sphingobacterium multivorum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| palmitoyl-CoA + L-serine | - |
Sphingobacterium multivorum | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
| palmitoyl-CoA + L-serine | - |
Sphingobacterium spiritivorum | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
| palmitoyl-CoA + L-serine | - |
Bacteriovorax stolpii | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | gel filtration | Sphingobacterium multivorum |
| homodimer | gel filtration | Sphingobacterium spiritivorum |
| homodimer | gel filtration | Bacteriovorax stolpii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| serine palmitoyltransferase | - |
Sphingobacterium multivorum |
| serine palmitoyltransferase | - |
Sphingobacterium spiritivorum |
| serine palmitoyltransferase | - |
Bacteriovorax stolpii |
| SPT | - |
Sphingobacterium multivorum |
| SPT | - |
Sphingobacterium spiritivorum |
| SPT | - |
Bacteriovorax stolpii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
palmitoyl-CoA | - |
Bacteriovorax stolpii | |
| 0.12 | - |
palmitoyl-CoA | - |
Sphingobacterium multivorum | |
| 0.15 | - |
palmitoyl-CoA | - |
Sphingobacterium spiritivorum | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Sphingobacterium spiritivorum |
| 7 | 8 | - |
Bacteriovorax stolpii |
| 7.4 | 8 | - |
Sphingobacterium multivorum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
protein denatures below pH 7.2 | Sphingobacterium multivorum |
| 6.8 | 8.5 | 90% activity | Sphingobacterium spiritivorum |
| 6.8 | 8.5 | 90% activity | Bacteriovorax stolpii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Sphingobacterium multivorum | |
| pyridoxal 5'-phosphate | - |
Sphingobacterium spiritivorum | |
| pyridoxal 5'-phosphate | - |
Bacteriovorax stolpii | |
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