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Literature summary for 2.3.1.5 extracted from
- Bacillus cereus strain 10-L-2 produces two arylamine N-acetyltransferases that transform 4-phenylenediamine into 4-aminoacetanilide (2007), J. Biosci. Bioeng., 103, 147-154.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 26% remaining activity isoenzyme Nat-b; 30% remaining activity isoenzyme NAT-a | Bacillus cereus |  |
| Co2+ | 39% remaining activity isoenzyme NAT-a | Bacillus cereus | |
| Cu2+ | 30% remaining activity isoenzyme Nat-b | Bacillus cereus | |
| Hg2+ | 11% remaining activity isoenzyme Nat-b; 36% remaining activity isoenzyme NAT-a | Bacillus cereus | |
| iodoacetic acid | 13% remaining activity | Bacillus cereus | |
| Zn2+ | 39% remaining activity isoenzyme Nat-b; 41% remaining activity isoenzyme NAT-a | Bacillus cereus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.2 | - |
4-phenylenediamine | - |
Bacillus cereus | |
| 3.2 | - |
N,N-1,4-phenylenediamine | Vmax: 0.17 micromol/min/mg | Bacillus cereus | |
| 5.4 | - |
4-phenylenediamine | - |
Bacillus cereus | |
| 5.4 | - |
N,N-1,4-phenylenediamine | Vmax: 2.1 micromol/min/mg | Bacillus cereus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27500 | - |
NAT-b, determined by SDS-PAGE | Bacillus cereus |
| 27500 | - |
SDS-PAGE, isoenzyme Nat-b | Bacillus cereus |
| 31000 | - |
NAT-a, determined by SDS-PAGE | Bacillus cereus |
| 31000 | - |
SDS-PAGE, isoenzyme NAT-a | Bacillus cereus |
| 35000 | - |
gel filtration, isoenzyme NAT-a | Bacillus cereus |
| 35000 | - |
gel filtration, isoenzyme Nat-b | Bacillus cereus |
| 35000 | - |
NAT-a, determined by gel filtration | Bacillus cereus |
| 35000 | - |
NAT-b, determined by gel filtration | Bacillus cereus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + an N-hydroxyarylamine | Bacillus cereus | - |
CoA + an N-acetoxyarylamine | - |
? | |
| acetyl-CoA + an N-hydroxyarylamine | Bacillus cereus 10-L-2 | - |
CoA + an N-acetoxyarylamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
- |
- |
| Bacillus cereus 10-L-2 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| NAT is purified from the extract of Bacillus cereus cultures using ammonium sulfate precipitation, a DEAE-cellulose, a DEAE-Toyopearl and a hydrophobic POROS 20PE column | Bacillus cereus |
| using DE52 column chromatography, DEAE-Toypearl columns and hydrophobic POROS 20PE columns | Bacillus cereus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
relative activity isoenzyme NAT-a (substrate): 12% (aniline), 90% (2-phenylendiamine), 0% (3-phenylendiamine), 100% (4-phenylendiamine), 72% (2-aminophenol), 33% (3-aminophenol), 94% (4-aminophenol), 0% (2-aminobenzoic acid), 122% (3-aminobenzoic acid), 19% (4-aminobenzoic acid), 0% (2-nitroaniline), 2% (3-nitroaniline), 0% (4-nitroaniline), 18% (2-chloraniline), 48% (3-chloraniline), 18% (4-chloraniline), 0% (2-toluidine), 21% (3-toluidine), 80% (4-toluidine), 46% (sulfamethazine), 90% (4-aminosalicylic acid), 550% (5-aminosalicylic acid), 0% (4-aminoacetanilide) | Bacillus cereus |
| additional information | - |
relative activity isoenzyme Nat-b (substrate): 24% (aniline), 80% (2-phenylendiamine), 0% (3-phenylendiamine), 100% (4-phenylendiamine), 36% (2-aminophenol), 56% (3-aminophenol), 48% (4-aminophenol), 0% (2-aminobenzoic acid), 105% (3-aminobenzoic acid), 26% (4-aminobenzoic acid), 3% (2-nitroaniline), 6% (3-nitroaniline), 0% (4-nitroaniline), 12% (2-chloraniline), 48% (3-chloraniline), 21% (4-chloraniline), 0% (2-toluidine), 28% (3-toluidine), 40% (4-toluidine), 0% (sulfamethazine), 100% (4-aminosalicylic acid), 510% (5-aminosalicylic acid), 0% (4-aminoacetanilide) | Bacillus cereus |
| 0.19 | - |
after streptomycin sulfate treatment | Bacillus cereus |
| 0.25 | - |
cell extract | Bacillus cereus |
| 0.38 | - |
purification step ammonium sulfate precipitation | Bacillus cereus |
| 7 | - |
purification step POROS 20PE column | Bacillus cereus |
| 7.3 | - |
purification step DE52-cellulose column | Bacillus cereus |
| 9 | - |
purification step DE52-cellulose column | Bacillus cereus |
| 9 | - |
purification step POROS 20PE column | Bacillus cereus |
| 26 | - |
purification step DEAE Toyopearl column | Bacillus cereus |
| 27 | - |
purification step DEAE Toyopearl column | Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + 2-aminobenzoic acid | relative activity: 0% | Bacillus cereus | CoA + N-acetyl-2-aminobenzoic acid | - |
? | |
| acetyl-CoA + 2-aminophenol | relative activity: 36% | Bacillus cereus | CoA + N-acetyl-2-aminophenol | - |
? | |
| acetyl-CoA + 2-aminophenol | relative activity: 72% | Bacillus cereus | CoA + N-acetyl-2-aminophenol | - |
? | |
| acetyl-CoA + 2-chloroaniline | relative activity: 12% | Bacillus cereus | CoA + N-acetyl-2-chloroaniline | - |
? | |
| acetyl-CoA + 2-chloroaniline | relative activity: 18% | Bacillus cereus | CoA + N-acetyl-2-chloroaniline | - |
? | |
| acetyl-CoA + 2-nitroaniline | relative activity: 0% | Bacillus cereus | CoA + N-acetyl-2-nitroaniline | - |
? | |
| acetyl-CoA + 2-nitroaniline | relative activity: 3% | Bacillus cereus | CoA + N-acetyl-2-nitroaniline | - |
? | |
| acetyl-CoA + 2-phenylendiamine | relative activity: 80% | Bacillus cereus | CoA + ? | - |
? | |
| acetyl-CoA + 2-phenylendiamine | relative activity: 90% | Bacillus cereus | CoA + ? | - |
? | |
| acetyl-CoA + 2-toluidine | relative activity: 0% | Bacillus cereus | CoA + N-acetyl-2-toluidine | - |
? | |
| acetyl-CoA + 3-aminobenzoic acid | relative activity: 105% | Bacillus cereus | CoA + N-acetyl-3-aminobenzoic acid | - |
? | |
| acetyl-CoA + 3-aminobenzoic acid | relative activity: 122% | Bacillus cereus | CoA + N-acetyl-3-aminobenzoic acid | - |
? | |
| acetyl-CoA + 3-aminobenzoic acid | relative activity: 105% | Bacillus cereus 10-L-2 | CoA + N-acetyl-3-aminobenzoic acid | - |
? | |
| acetyl-CoA + 3-aminobenzoic acid | relative activity: 122% | Bacillus cereus 10-L-2 | CoA + N-acetyl-3-aminobenzoic acid | - |
? | |
| acetyl-CoA + 3-aminophenol | relative activity: 33% | Bacillus cereus | CoA + N-acetyl-3-aminophenol | - |
? | |
| acetyl-CoA + 3-aminophenol | relative activity: 56% | Bacillus cereus | CoA + N-acetyl-3-aminophenol | - |
? | |
| acetyl-CoA + 3-chloraniline | relative activity: 48% | Bacillus cereus | CoA + N-acetyl-3-chloroaniline | - |
? | |
| acetyl-CoA + 3-nitroaniline | relative activity: 2% | Bacillus cereus | CoA + N-acetyl-3-nitroaniline | - |
? | |
| acetyl-CoA + 3-nitroaniline | relative activity: 6% | Bacillus cereus | CoA + N-acetyl-3-nitroaniline | - |
? | |
| acetyl-CoA + 3-phenylendiamine | relative activity: 0% | Bacillus cereus | CoA + ? | - |
? | |
| acetyl-CoA + 3-toluidine | relative activity: 21% | Bacillus cereus | CoA + N-acetyl-3-toluidine | - |
? | |
| acetyl-CoA + 3-toluidine | relative activity: 28% | Bacillus cereus | CoA + N-acetyl-3-toluidine | - |
? | |
| acetyl-CoA + 4-aminoacetanilide | relative activity: 0% | Bacillus cereus | CoA + N-acetyl-4-aminoacetanilide | - |
? | |
| acetyl-CoA + 4-aminobenzoic acid | relative activity: 19% | Bacillus cereus | CoA + N-acetyl-4-aminobenzoic acid | - |
? | |
| acetyl-CoA + 4-aminobenzoic acid | relative activity: 26% | Bacillus cereus | CoA + N-acetyl-4-aminobenzoic acid | - |
? | |
| acetyl-CoA + 4-aminophenol | relative activity: 48% | Bacillus cereus | CoA + N-acetyl-4-aminophenol | - |
? | |
| acetyl-CoA + 4-aminophenol | relative activity: 94% | Bacillus cereus | CoA + N-acetyl-4-aminophenol | - |
? | |
| acetyl-CoA + 4-aminosalicylic acid | relative activity: 100% | Bacillus cereus | CoA + N-acetyl-4-aminosalicylic acid | - |
? | |
| acetyl-CoA + 4-aminosalicylic acid | relative activity: 90% | Bacillus cereus | CoA + N-acetyl-4-aminosalicylic acid | - |
? | |
| acetyl-CoA + 4-chloroaniline | relative activity: 18% | Bacillus cereus | CoA + N-acetyl-4-chloroaniline | - |
? | |
| acetyl-CoA + 4-chloroaniline | relative activity: 21% | Bacillus cereus | CoA + N-acetyl-4-chloroaniline | - |
? | |
| acetyl-CoA + 4-nitroaniline | relative activity: 0% | Bacillus cereus | CoA + N-acetyl-4-nitroaniline | - |
? | |
| acetyl-CoA + 4-nitroaniline | relative activity: 0% | Bacillus cereus 10-L-2 | CoA + N-acetyl-4-nitroaniline | - |
? | |
| acetyl-CoA + 4-phenylenediamine | - |
Bacillus cereus | CoA + 4-aminoacetanilide | - |
? | |
| acetyl-CoA + 4-toluidine | relative activity: 40% | Bacillus cereus | CoA + N-acetyl-4-toluidine | - |
? | |
| acetyl-CoA + 4-toluidine | relative activity: 80% | Bacillus cereus | CoA + N-acetyl-4-toluidine | - |
? | |
| acetyl-CoA + 5-aminosalicylic acid | - |
Bacillus cereus | CoA + N-acetyl-5-aminosalicylic acid | - |
? | |
| acetyl-CoA + 5-aminosalicylic acid | relative activity: 510% | Bacillus cereus | CoA + N-acetyl-5-aminosalicylic acid | - |
? | |
| acetyl-CoA + 5-aminosalicylic acid | relative activity: 550% | Bacillus cereus | CoA + N-acetyl-5-aminosalicylic acid | - |
? | |
| acetyl-CoA + an N-hydroxyarylamine | - |
Bacillus cereus | CoA + an N-acetoxyarylamine | - |
? | |
| acetyl-CoA + an N-hydroxyarylamine | - |
Bacillus cereus 10-L-2 | CoA + an N-acetoxyarylamine | - |
? | |
| acetyl-CoA + aniline | relative activity: 12% | Bacillus cereus | CoA + N-acetyl-aniline | - |
? | |
| acetyl-CoA + aniline | relative activity: 24% | Bacillus cereus | CoA + N-acetyl-aniline | - |
? | |
| acetyl-CoA + N,N-1,4-phenylenediamine | relative activity: 100% | Bacillus cereus | CoA + ? | - |
? | |
| acetyl-CoA + sulfamethazine | - |
Bacillus cereus | CoA + N-4-acetylsulfamethazine | - |
? | |
| acetyl-CoA + sulfamethazine | - |
Bacillus cereus 10-L-2 | CoA + N-4-acetylsulfamethazine | - |
? | |
| acetyl-CoA + sulfamethazine | relative activity: 0% | Bacillus cereus | CoA + N-acetyl-sulfamethazine | - |
? | |
| acetyl-CoA + sulfamethazine | relative activity: 46% | Bacillus cereus | CoA + N-acetyl-sulfamethazine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| arylamine N-acetyltransferase | - |
Bacillus cereus |
| N-acetyltransferase a | - |
Bacillus cereus |
| N-acetyltransferase b | - |
Bacillus cereus |
| NAT-a | - |
Bacillus cereus |
| NAT-b | - |
Bacillus cereus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bacillus cereus |
| 30 | - |
activity assay | Bacillus cereus |
| 37 | - |
enzyme assayed at various temperatures | Bacillus cereus |
| 37 | - |
optimum temperature | Bacillus cereus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
30% activity | Bacillus cereus |
| 42 | - |
50% activity | Bacillus cereus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
incubation at 42°C for 10 min: 30% remaining activity isoenzyme NAT-a | Bacillus cereus |
| additional information | - |
incubation at 42°C for 10 min: 50% remaining activity isoenzyme Nat-b | Bacillus cereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | - |
assay at | Bacillus cereus |
| 7.1 | - |
activity assay | Bacillus cereus |
| 7.5 | - |
pH-optimum | Bacillus cereus |
| 7.5 | - |
enzyme assayed at various pHs at 30°C | Bacillus cereus |
| 8 | - |
pH-optimum | Bacillus cereus |
| 8 | - |
enzyme assayed at various pHs at 30°C | Bacillus cereus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | 80% activity | Bacillus cereus |
| 7 | 9.5 | 80% activity | Bacillus cereus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | incubation overnight at pH 6.5-9.5: 80% remaining activity isoenzyme Nat-b | Bacillus cereus |
| 7 | 9.5 | incubation overnight at pH 7-9.5: 80% remaining activity isoenzyme NAT-a | Bacillus cereus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Bacillus cereus | |
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