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Literature summary for 2.3.1.5 extracted from

  • Savulescu, M.R.; Mushtaq, A.; Josephy, P.D.
    Screening and characterizing human NAT2 variants (2005), Methods Enzymol., 400, 192-215.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
isozyme NAT2, expression in Escherichia coli strain DJ2002 Homo sapiens

Protein Variants

Protein Variants Comment Organism
E8G site-directed mutagenesis, the NAT2 mutant shows reduced activity compared to the wild-type enzyme Homo sapiens
F192Y site-directed mutagenesis, the NAT2 mutant shows highly reduced activity compared to the wild-type enzyme Homo sapiens
H43R site-directed mutagenesis, the NAT2 mutant shows reduced activity compared to the wild-type enzyme Homo sapiens
I32V site-directed mutagenesis, the NAT2 mutant shows reduced activity compared to the wild-type enzyme Homo sapiens
K13R site-directed mutagenesis, the NAT2 mutant shows reduced activity compared to the wild-type enzyme Homo sapiens
K141E site-directed mutagenesis, the NAT2 mutant shows reduced activity compared to the wild-type enzyme Homo sapiens
L239F site-directed mutagenesis, the NAT2 mutant shows reduced activity compared to the wild-type enzyme Homo sapiens
L69P site-directed mutagenesis, the NAT2 mutant shows highly reduced activity compared to the wild-type enzyme Homo sapiens
L74P site-directed mutagenesis, the NAT2 mutant shows reduced activity compared to the wild-type enzyme Homo sapiens
additional information NAT2 random mutagenesis, developement of a system in which the activation of mutagens by recombinant human NAT 2, expressed in Escherichia coli, can be detected by the appearance of LacZ revertants, screening for variants of the human NAT2 sequence with altered activity, detailed overview Homo sapiens
Q133R site-directed mutagenesis, the NAT2 mutant shows reduced activity compared to the wild-type enzyme Homo sapiens
S102C site-directed mutagenesis, the NAT2 mutant shows reduced activity compared to the wild-type enzyme Homo sapiens
T250P site-directed mutagenesis, the NAT2 mutant shows reduced activity compared to the wild-type enzyme Homo sapiens
Y190C site-directed mutagenesis, the NAT2 mutant shows highly reduced activity compared to the wild-type enzyme Homo sapiens
Y190F site-directed mutagenesis, the NAT2 mutant shows reduced activity compared to the wild-type enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + 2-aminofluorene Homo sapiens
-
CoA + 2-acetylaminofluorene
-
?
additional information Homo sapiens NAT plays a key role in the metabolic activation of aromatic amine and nitroaromatic mutagens to electrophilic reactive intermediates ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P11245 isozyme NAT2; isozyme NAT2
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity of recombinant wild-type and mutant NAT2, overview Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 2-aminofluorene
-
Homo sapiens CoA + 2-acetylaminofluorene
-
?
acetyl-CoA + 2-aminofluorene interactions and substrate binding structure, overview Homo sapiens CoA + 2-acetylaminofluorene
-
?
additional information NAT plays a key role in the metabolic activation of aromatic amine and nitroaromatic mutagens to electrophilic reactive intermediates Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
More modeling and analysis of the three-dimensional structures of human NAT enzymes, overview, sequence and structure comparisons, NAT2 model structure possesses three structural domains Homo sapiens

Synonyms

Synonyms Comment Organism
NAT
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
Homo sapiens