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Literature summary for 2.3.1.5 extracted from

  • Suzuki, H.; Ohnishi, Y.; Horinouchi, S.
    Arylamine N-acetyltransferase responsible for acetylation of 2-aminophenols in Streptomyces griseus (2007), J. Bacteriol., 189, 2155-2159.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.5

Cloned(Commentary)

Cloned (Comment) Organism
gene natA, DNA and amino acid sequence determination and analysis, genetic structure, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3) Streptomyces griseus

Protein Variants

Protein Variants Comment Organism
additional information construction of a natA disruption mutant that is catalytically inactive Streptomyces griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.81
-
 2-amino-4-chlorophenol pH 7.0, 30°C, recombinant enzyme Streptomyces griseus
1.4
-
 2-Aminophenol pH 7.0, 30°C, recombinant enzyme Streptomyces griseus
1.9
-
 2-amino-4-methylphenol pH 7.0, 30°C, recombinant enzyme Streptomyces griseus
2
-
 3-Amino-4-hydroxybenzoic acid pH 7.0, 30°C, recombinant enzyme Streptomyces griseus
2.6
-
 3-amino-4-hydroxybenzaldehyde pH 7.0, 30°C, recombinant enzyme Streptomyces griseus
12
-
 3-amino-4-hydroxybenzenesulfonic acid pH 7.0, 30°C, recombinant enzyme Streptomyces griseus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Streptomyces griseus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 1 * 34000, recombinant enzyme, SDS-PAGE Streptomyces griseus
40000
-
 recombinant enzyme, gel filtration Streptomyces griseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + 2-aminophenol Streptomyces griseus
-
 CoA + 2-acetylaminophenol
-
 ?
acetyl-CoA + 2-aminophenol Streptomyces griseus IFO 13350
-
 CoA + 2-acetylaminophenol
-
 ?
acetyl-CoA + 3-amino-4-hydroxybenzaldehyde Streptomyces griseus an intermediate of grixazone synthesis CoA + 3-acetylamino-4-hydroxybenzaldehyde
-
 ?
acetyl-CoA + 3-amino-4-hydroxybenzaldehyde Streptomyces griseus IFO 13350 an intermediate of grixazone synthesis CoA + 3-acetylamino-4-hydroxybenzaldehyde
-
 ?
acetyl-CoA + 3-amino-4-hydroxybenzoic acid Streptomyces griseus an intermediate of grixazone synthesis CoA + 3-acetylamino-4-hydroxybenzoic acid
-
 ?
acetyl-CoA + 5-aminosalicylic acid Streptomyces griseus
-
 CoA + 5-acetylaminosalicylic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces griseus A2V7M5 gene natA
-
Streptomyces griseus IFO 13350 A2V7M5 gene natA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Streptomyces griseus

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine Cys69, His108, and Asp125 form the catalytic triad Streptomyces griseus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Streptomyces griseus
0.29
-
 purified recombinant enzyme, with substrate 3-amino-4-hydroxybenzenesulfonic acid Streptomyces griseus
0.33
-
 purified recombinant enzyme, with substrate 3-amino-4-hydroxybenzaldehyde Streptomyces griseus
0.46
-
 purified recombinant enzyme, with substrate 2-amino-4-chlorophenol Streptomyces griseus
0.47
-
 purified recombinant enzyme, with substrate 2-aminophenol Streptomyces griseus
1.1
-
 purified recombinant enzyme, with substrate 3-amino-4-hydroxybenzoic acid or 2-amino-4-methylphenol Streptomyces griseus

Storage Stability

Storage Stability Organism
4°C, purified recombinant enzyme in 50 mM sodium phosphate, pH 7.0, 0.15 M NaCl, and 7.5 mM 2-mercaptoethanol, 2 weeks, stable Streptomyces griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 2-amino-4-chlorophenol
-
 Streptomyces griseus CoA + 2-acetylamino-4-chlorophenol
-
 ?
acetyl-CoA + 2-amino-4-methylphenol best substrate Streptomyces griseus CoA + 2-acetylamino-4-methylphenol
-
 ?
acetyl-CoA + 2-aminophenol
-
 Streptomyces griseus CoA + 2-acetylaminophenol
-
 ?
acetyl-CoA + 2-aminophenol
-
 Streptomyces griseus IFO 13350 CoA + 2-acetylaminophenol
-
 ?
acetyl-CoA + 3-amino-4-hydroxybenzaldehyde
-
 Streptomyces griseus CoA + 3-acetylamino-4-hydroxybenzaldehyde
-
 ?
acetyl-CoA + 3-amino-4-hydroxybenzaldehyde an intermediate of grixazone synthesis Streptomyces griseus CoA + 3-acetylamino-4-hydroxybenzaldehyde
-
 ?
acetyl-CoA + 3-amino-4-hydroxybenzaldehyde
-
 Streptomyces griseus IFO 13350 CoA + 3-acetylamino-4-hydroxybenzaldehyde
-
 ?
acetyl-CoA + 3-amino-4-hydroxybenzaldehyde an intermediate of grixazone synthesis Streptomyces griseus IFO 13350 CoA + 3-acetylamino-4-hydroxybenzaldehyde
-
 ?
acetyl-CoA + 3-amino-4-hydroxybenzenesulfonic acid
-
 Streptomyces griseus CoA + 3-acetylamino-4-hydroxybenzenesulfonic acid
-
 ?
acetyl-CoA + 3-amino-4-hydroxybenzoic acid best substrate Streptomyces griseus CoA + 3-acetylamino-4-hydroxybenzoic acid
-
 ?
acetyl-CoA + 3-amino-4-hydroxybenzoic acid an intermediate of grixazone synthesis Streptomyces griseus CoA + 3-acetylamino-4-hydroxybenzoic acid
-
 ?
acetyl-CoA + 3-amino-4-hydroxybenzoic acid best substrate Streptomyces griseus IFO 13350 CoA + 3-acetylamino-4-hydroxybenzoic acid
-
 ?
acetyl-CoA + 4-amino-3-hydroxybenzoic acid low activity Streptomyces griseus CoA + 4-acetylamino-3-hydroxybenzoic acid
-
 ?
acetyl-CoA + 5-aminosalicylic acid
-
 Streptomyces griseus CoA + 5-acetylaminosalicylic acid
-
 ?
additional information substrate specificity, product identification by LC-MS, 4-aminobenzoic acid, 3-aminobenzoic acid, and aniline are poor substrates, overview Streptomyces griseus ?
-
 ?
additional information substrate specificity, product identification by LC-MS, 4-aminobenzoic acid, 3-aminobenzoic acid, and aniline are poor substrates, overview Streptomyces griseus IFO 13350 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 34000, recombinant enzyme, SDS-PAGE Streptomyces griseus

Synonyms

Synonyms Comment Organism
NAT
-
 Streptomyces griseus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Streptomyces griseus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.27
-
 2-amino-4-chlorophenol pH 7.0, 30°C, recombinant enzyme Streptomyces griseus
0.29
-
 3-amino-4-hydroxybenzaldehyde pH 7.0, 30°C, recombinant enzyme Streptomyces griseus
0.41
-
 2-Aminophenol pH 7.0, 30°C, recombinant enzyme Streptomyces griseus
1.1
-
 3-amino-4-hydroxybenzenesulfonic acid pH 7.0, 30°C, recombinant enzyme Streptomyces griseus
1.3
-
 3-Amino-4-hydroxybenzoic acid pH 7.0, 30°C, recombinant enzyme Streptomyces griseus
1.3
-
 2-amino-4-methylphenol pH 7.0, 30°C, recombinant enzyme Streptomyces griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Streptomyces griseus

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Streptomyces griseus