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Literature summary for 2.3.1.5 extracted from

  • Andres, H.H.; Kolb, H.J.; Weiss, L.
    Purification and physical-chemical properties of acetyl-CoA:arylamine N-acetyltransferase from pigeon liver (1983), Biochim. Biophys. Acta, 746, 182-192.
    View publication on PubMed
Search for more literature for 2.3.1.5

General Stability

General Stability Organism
dithiothreitol stabilizes Columba sp.
EDTA stabilizes Columba sp.
repeated freeze-thawing inactivates Columba sp.

Inhibitors

Inhibitors Comment Organism Structure
DTNB
-
 Columba sp.
methopterin kinetics Columba sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid analysis Columba sp.
31900
-
 1 * 31900, SDS-PAGE Columba sp.
32900
-
 high-speed sedimentation equilibrium analysis Columba sp.
33000
-
 gel filtration Columba sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + an arylamine Columba sp. constitutive enzyme CoA + a N-acetylarylamine
-
 ?

Organism

Organism UniProt Comment Textmining
Columba sp.
-
 pigeon
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Columba sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Columba sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.02
-
-
 Columba sp.

Storage Stability

Storage Stability Organism
-70°C, frozen at neutral pH in liquid nitrogen, no loss of activity over a long-time range Columba sp.
4°C, several days in various buffer systems at pH 6.0-8.5 Columba sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 4-nitroaniline
-
 Columba sp. CoA + N-acetyl-4-nitroaniline
-
 ?
acetyl-CoA + an arylamine constitutive enzyme Columba sp. CoA + a N-acetylarylamine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 31900, SDS-PAGE Columba sp.
More enzyme forms dimer under conditions of catalysis Columba sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 4.8 Columba sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8.5 in several buffer systems, at 4°C, several days stable Columba sp.