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Literature summary for 2.3.1.48 extracted from
- Akt phosphorylation of p300 at Ser-1834 is essential for its histone acetyltransferase and transcriptional activity (2005), Mol. Cell. Biol., 25, 6592-6602.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| T7-coupled in vitro transcription and translation of FLAG-tagged enzyme | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S1834A | site-directed mutagenesis, the mutant shows no histone acetylation and ICAM-1 gene expression, overview | Homo sapiens |
| S1834E | site-directed mutagenesis, the mutant shows no histone acetylation and ICAM-1 gene expression, overview | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Homo sapiens | 5634 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| histone + acetyl-CoA | Homo sapiens | phosphorylation of p300 at Ser1834 by the kinase Akt is essential for its histone acetyltransferase and transcriptional activity | acetyl-histone + CoA | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | phosphorylation of p300 at Ser1834, in vitro and in vivo, by the kinase Akt is essential for its histone acetyltransferase and transcriptional activity, Akt phosphorylation of Ser1834 enhances the transcriptional activity of p300 by increasing its promoter recruitment, histone acetylation, and enhanceosome formation on the ICAM-1 promoter, molecular mechanism by which Akt promotes the transcriptional potential of p300, overview, nuclear Akt interacts with and phosphorylates p300 in response to TNF-alpha | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| A-549 cell | - |
Homo sapiens | - |
| U-937 cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| histone + acetyl-CoA | - |
Homo sapiens | acetyl-histone + CoA | - |
? | |
| histone + acetyl-CoA | phosphorylation of p300 at Ser1834 by the kinase Akt is essential for its histone acetyltransferase and transcriptional activity | Homo sapiens | acetyl-histone + CoA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HAT | - |
Homo sapiens |
| p300 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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