Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a target for development of drugs against mycobac-terial pathogens | Mycolicibacterium smegmatis |
Cloned (Comment) | Organism |
---|---|
gene bioF, the enzyme coding gene is located between genes bioA(MSMEG 3188) and bioD (MSMEG 3190), recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3) | Mycolicibacterium smegmatis |
Crystallization (Comment) | Organism |
---|---|
the crystal structure of MsBioF is solved by molecular replacement using the structure of EcAONS, PDB code 1BS0, as the search model | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pimeloyl-[acyl-carrier protein] + L-alanine | Mycolicibacterium smegmatis | - |
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] | - |
? | |
pimeloyl-[acyl-carrier protein] + L-alanine | Mycolicibacterium smegmatis ATCC 700084 | - |
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | A0QX65 | gene bioF | - |
Mycolicibacterium smegmatis ATCC 700084 | A0QX65 | gene bioF | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pimeloyl-[acyl-carrier protein] + L-alanine | - |
Mycolicibacterium smegmatis | 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] | - |
? | |
pimeloyl-[acyl-carrier protein] + L-alanine | - |
Mycolicibacterium smegmatis ATCC 700084 | 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | the structure reveals an incomplete dimer, three-dimensional structure of apoenzyme, overview | Mycolicibacterium smegmatis |
Synonyms | Comment | Organism |
---|---|---|
7-keto-8-aminopelargonic acid synthase | - |
Mycolicibacterium smegmatis |
BioF | - |
Mycolicibacterium smegmatis |
KAPA synthase | - |
Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on, binding site residues His129, Lys235, and His200 are essential for MsBioF activity in vivo | Mycolicibacterium smegmatis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is essential for mycobacterial growth and plays a key role as the gatekeeper of the biotin biosynthesis pathway, catalyzing the first committed step, decarboxylative condensation of L-alanine with pimeloyl-CoA to form 7-oxo-8-aminopelargonic acid | Mycolicibacterium smegmatis |
additional information | the structure reveals an incomplete dimer, and the active site organization is similar to, but distinct from Escherichia coli 8-amino-7-oxononanoate synthase (EcAONS). Pyridoxal 5'-phosphate binding site residues His129, Lys235, and His200 are essential for MsBioF activity in vivo and residue Glu171 plays an important, but not essential role in MsBioF activity. The N-terminus (residues 1-37) is also essential for MsBioF activity in vivo, structure homology modeling and structure-function analysis of MsBioF, overview | Mycolicibacterium smegmatis |