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Literature summary for 2.3.1.47 extracted from

  • Kubota, T.; Izumi, Y.
    Detection and characterization of a thermophilic biotin biosynthetic enzyme, 7-keto-8-aminopelargonic acid synthase, from various thermophiles (2012), Biosci. Biotechnol. Biochem., 76, 685-690.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
GST-fusion protein expressed in Escherichia coli JM109 Escherichia coli
GST-fusion protein expressed in Escherichia coli JM109 Symbiobacterium thermophilum
GST-fusion protein expressed in Escherichia coli JM109 Acetivibrio thermocellus
GST-fusion protein expressed in Escherichia coli JM109 Aquifex aeolicus
GST-fusion protein expressed in Escherichia coli JM109 Geobacillus kaustophilus
His-tagged protein expressed in Escherichia coli Rosetta-gami (DE3) Thermosynechococcus vestitus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
pimeloyl-CoA pH 6.0, 60°C Aquifex aeolicus
0.02
-
pimeloyl-CoA pH 6.0, 60°C Geobacillus kaustophilus
0.025
-
pimeloyl-CoA pH not specified in the publication, temperature not specified in the publication Escherichia coli
0.03
-
pimeloyl-CoA pH 6.0, 60°C Symbiobacterium thermophilum
0.09
-
L-alanine pH 6.0, 60°C Geobacillus kaustophilus
0.23
-
L-alanine pH 6.0, 60°C Aquifex aeolicus
0.5
-
L-alanine pH not specified in the publication, temperature not specified in the publication Escherichia coli
2.28
-
L-alanine pH 6.0, 60°C Symbiobacterium thermophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
x * 44000, SDS-PAGE Escherichia coli
44000
-
x * 44000, SDS-PAGE Geobacillus kaustophilus
45000
-
x * 45000, SDS-PAGE Acetivibrio thermocellus
45000
-
x * 45000, SDS-PAGE Aquifex aeolicus
45000
-
x * 45000, SDS-PAGE Thermosynechococcus vestitus
46000
-
x * 46000, SDS-PAGE Symbiobacterium thermophilum

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-
Acetivibrio thermocellus ATCC 27405
-
-
-
Aquifex aeolicus
-
-
-
Escherichia coli
-
-
-
Geobacillus kaustophilus
-
-
-
Geobacillus kaustophilus JCM 12893
-
-
-
Symbiobacterium thermophilum
-
-
-
Symbiobacterium thermophilum IAM14863
-
-
-
Thermosynechococcus vestitus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography Acetivibrio thermocellus
Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography, gel filtration Aquifex aeolicus
Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography, heat treatment with centrifugation Symbiobacterium thermophilum
Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography, ion exchange chromatography Escherichia coli
Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography, ion exchange chromatography Geobacillus kaustophilus
immobilized metal ion affinity chromatography (Ni2+), His-tag cleavage, immobilized metal ion affinity chromatography (Ni2+), gel filtration Thermosynechococcus vestitus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.032
-
glycine, pimeloyl-CoA, pH 6.0, 60°C Symbiobacterium thermophilum
0.071
-
L-alanine, pimeloyl-CoA, pH 6.0, 60°C Geobacillus kaustophilus
0.078
-
L-alanine, pimeloyl-CoA, pH 6.0, 60°C Symbiobacterium thermophilum
0.272
-
L-alanine, acetyl-CoA, pH 6.0, 60°C Symbiobacterium thermophilum
0.897
-
glycine, acetyl-CoA, pH 6.0, 60°C Symbiobacterium thermophilum
2.46
-
L-alanine, pimeloyl-CoA, pH 6.0, 60°C Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycine + acetyl-CoA
-
Symbiobacterium thermophilum ?
-
?
glycine + acetyl-CoA
-
Symbiobacterium thermophilum IAM14863 ?
-
?
glycine + pimeloyl-CoA
-
Symbiobacterium thermophilum ?
-
?
glycine + pimeloyl-CoA
-
Symbiobacterium thermophilum IAM14863 ?
-
?
L-alanine + acetyl-CoA
-
Symbiobacterium thermophilum ?
-
?
L-alanine + acetyl-CoA
-
Symbiobacterium thermophilum IAM14863 ?
-
?
L-alanine + pimeloyl-CoA
-
Escherichia coli 8-amino-7-oxononanoate + CoA + CO2
-
?
L-alanine + pimeloyl-CoA
-
Acetivibrio thermocellus 8-amino-7-oxononanoate + CoA + CO2
-
?
L-alanine + pimeloyl-CoA
-
Thermosynechococcus vestitus 8-amino-7-oxononanoate + CoA + CO2
-
?
L-alanine + pimeloyl-CoA no activity observed with acetyl-CoA, no activity observed with glycine or L-serine Aquifex aeolicus 8-amino-7-oxononanoate + CoA + CO2
-
?
L-alanine + pimeloyl-CoA no activity observed with acetyl-CoA, no activity observed with glycine or L-serine Geobacillus kaustophilus 8-amino-7-oxononanoate + CoA + CO2
-
?
L-alanine + pimeloyl-CoA no activity observed with L-serine Symbiobacterium thermophilum 8-amino-7-oxononanoate + CoA + CO2
-
?
L-alanine + pimeloyl-CoA no activity observed with acetyl-CoA, no activity observed with glycine or L-serine Geobacillus kaustophilus JCM 12893 8-amino-7-oxononanoate + CoA + CO2
-
?
L-alanine + pimeloyl-CoA no activity observed with L-serine Symbiobacterium thermophilum IAM14863 8-amino-7-oxononanoate + CoA + CO2
-
?
L-alanine + pimeloyl-CoA
-
Acetivibrio thermocellus ATCC 27405 8-amino-7-oxononanoate + CoA + CO2
-
?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Acetivibrio thermocellus
? x * 45000, SDS-PAGE Aquifex aeolicus
? x * 45000, SDS-PAGE Thermosynechococcus vestitus
? x * 46000, SDS-PAGE Symbiobacterium thermophilum
? x * 44000, SDS-PAGE Escherichia coli
? x * 44000, SDS-PAGE Geobacillus kaustophilus

Synonyms

Synonyms Comment Organism
7-keto-8-aminopelargonic acid synthase
-
Escherichia coli
7-keto-8-aminopelargonic acid synthase
-
Symbiobacterium thermophilum
7-keto-8-aminopelargonic acid synthase
-
Acetivibrio thermocellus
7-keto-8-aminopelargonic acid synthase
-
Aquifex aeolicus
7-keto-8-aminopelargonic acid synthase
-
Thermosynechococcus vestitus
7-keto-8-aminopelargonic acid synthase
-
Geobacillus kaustophilus
8-amino-7-oxononanoate synthase
-
Escherichia coli
8-amino-7-oxononanoate synthase
-
Symbiobacterium thermophilum
8-amino-7-oxononanoate synthase
-
Acetivibrio thermocellus
8-amino-7-oxononanoate synthase
-
Aquifex aeolicus
8-amino-7-oxononanoate synthase
-
Thermosynechococcus vestitus
8-amino-7-oxononanoate synthase
-
Geobacillus kaustophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Escherichia coli
50 60
-
Symbiobacterium thermophilum
50 60
-
Acetivibrio thermocellus
50 60
-
Aquifex aeolicus
50 60
-
Thermosynechococcus vestitus
50 60
-
Geobacillus kaustophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable for one h Escherichia coli
55
-
stable for one h Symbiobacterium thermophilum
65
-
stable for one h Thermosynechococcus vestitus
70
-
stable for one h Acetivibrio thermocellus
75
-
stable for one h Geobacillus kaustophilus
93
-
stable for one h Aquifex aeolicus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Escherichia coli
pyridoxal 5'-phosphate
-
Symbiobacterium thermophilum
pyridoxal 5'-phosphate
-
Acetivibrio thermocellus
pyridoxal 5'-phosphate
-
Aquifex aeolicus
pyridoxal 5'-phosphate
-
Thermosynechococcus vestitus
pyridoxal 5'-phosphate
-
Geobacillus kaustophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.014
-
L-alanine pH 6.0, 60°C Symbiobacterium thermophilum
0.984
-
L-alanine pH 6.0, 60°C Geobacillus kaustophilus
1.5
-
pimeloyl-CoA pH 6.0, 60°C Symbiobacterium thermophilum
2.4
-
pimeloyl-CoA pH not specified in the publication, temperature not specified in the publication Escherichia coli
4.9
-
pimeloyl-CoA pH 6.0, 60°C Geobacillus kaustophilus
7.33
-
L-alanine pH 6.0, 60°C Aquifex aeolicus
213
-
pimeloyl-CoA pH 6.0, 60°C Aquifex aeolicus