Cloned (Comment) | Organism |
---|---|
gene bioF, DNA and amino acid sequence determination and analysis, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | functional complementation of an Escherichia coli mutant deficient in KAPA synthase reaction | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the recombinant KAPA synthase obeys Michaelis-Menten kinetics with respect to pimeloyl-CoA and L-Ala | Arabidopsis thaliana | |
0.0016 | - |
pimeloyl-CoA | pH 7.5, 30°C, recombinant enzyme | Arabidopsis thaliana | |
1.4 | - |
L-alanine | pH 7.5, 30°C, recombinant enzyme | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Arabidopsis thaliana | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
recombinant His-tagged enzyme, gel filtration | Arabidopsis thaliana |
52171 | - |
1 * 52171, amino acid sequence calculation | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | the enzyme catalyzes the first committed enzyme of the biotin synthesis pathway | ? | - |
? | |
pimeloyl-CoA + L-alanine | Arabidopsis thaliana | - |
8-amino-7-oxononanoate + CoA + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8GW43 | ecotype Wassilewskija | - |
Purification (Comment) | Organism |
---|---|
native enzyme partially by subcellular fractionation, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Arabidopsis thaliana |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] | via an AtbioF-PLP internal aldimine complex, i.e. a Schiff base formed between PLP and a Lys residue in the protein | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.09 | 0.1 | purified recombinant enzyme | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme catalyzes the first committed enzyme of the biotin synthesis pathway | Arabidopsis thaliana | ? | - |
? | |
pimeloyl-CoA + L-alanine | - |
Arabidopsis thaliana | 8-amino-7-oxononanoate + CoA + CO2 | - |
? | |
pimeloyl-CoA + L-alanine | the enzyme is strictly stereospecific for L-alanine | Arabidopsis thaliana | 8-amino-7-oxononanoate + CoA + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 52171, amino acid sequence calculation | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
7-keto-8-aminopelargonic acid synthase | - |
Arabidopsis thaliana |
AtbioF | - |
Arabidopsis thaliana |
KAPA synthase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
pimeloyl-CoA | pH 7.5, 30°C, recombinant enzyme | Arabidopsis thaliana | |
0.11 | - |
L-alanine | pH 7.5, 30°C, recombinant enzyme | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Arabidopsis thaliana |