Protein Variants | Comment | Organism |
---|---|---|
additional information | the enzyme is prone to aggregation under many stress conditions, resulting in a methionine limitation in Escherichia coli growth. Overexpression of MetA induces the greatest number of persisters at 42°C, which is correlated to an increased level of aggregated MetA. Substitution of the native metA gene on the Escherichia coli K-12 WE chromosome by a mutant gene encoding the stabilized MetA leads to reduction in persisters at the elevated temperature and in the presence of acetate, as well as lower aggregation of the mutated MetA. Decreased persister formation at 42°C is confirmed also in wild-type Escherichia coli K-12 W3110 and a fast-growing WErph+ mutant harboring the stabilized MetA. The frequency of persister formation is correlated to the aggregation of the MetA, more than 150fold increase in the frequency of persisters at 42°C result from an increased level of aggregated proteins. Stabilized MetA reduces persister formation in the presence of acetate | Escherichia coli |
General Stability | Organism |
---|---|
inherently unstable and prone to aggregation, the enzyme is prone to aggregation under many stress conditions, resulting in a methionine limitation in Escherichia coli growth | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + L-homoserine | Escherichia coli | - |
CoA + O-succinyl-L-homoserine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P07623 | gene metA | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the enzyme is prone to aggregation under many stress conditions, resulting in a methionine limitation in Escherichia coli growth | Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + L-homoserine | - |
Escherichia coli | CoA + O-succinyl-L-homoserine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
homoserine-O-succinyltransferase | - |
Escherichia coli |
MetA | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
44 | - |
complete aggregation of wild-tyype enzyyme at 44°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
succinyl-CoA | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | homoserine-o-succinyltransferase catalyzes the first unique step in the de novo methionine biosynthetic pathway | Escherichia coli |
additional information | the enzyme is prone to aggregation under many stress conditions, resulting in a methionine limitation in Escherichia coli growth | Escherichia coli |