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Literature summary for 2.3.1.45 extracted from

  • Corfield, A.P.; Ferreira do Amarral, C.; Wember, M.; Schauer, R.
    The metabolism of O-acyl-N-acylneuraminic acids. Biosynthesis of O-acylated sialic acids in bovine and equine submandibular glands (1976), Eur. J. Biochem., 68, 597-610.
    View publication on PubMed

General Stability

General Stability Organism
lyophilisation causes loss of 20-30% activity, storage in lyophilized stage leads to additional loss of 60% activity Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
CoA inhibition of membrane-bound enzyme Bos taurus
iodoacetate
-
Bos taurus
additional information no inhibition by MgCl2, CaCl2, MnCl2 Bos taurus
p-chloromercuribenzoate
-
Bos taurus
Triton X-100 no inhibition Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
acetyl-CoA cytosolic enzyme Bos taurus
0.12
-
acetyl-CoA membrane-bound enzyme Bos taurus
0.39
-
N-glycolylneuraminate cytosolic enzyme Bos taurus
0.5
-
N-acetylneuraminate cytosolic enzyme Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Bos taurus
-
-
microsome
-
Equus caballus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + sialic acid Bos taurus
-
CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate
-
?
acetyl-CoA + sialic acid Equus caballus
-
CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Equus caballus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
submandibular gland
-
Bos taurus
-
submandibular gland
-
Equus caballus
-

Storage Stability

Storage Stability Organism
0°C, 16 days, 55% loss of activity, membrane-bound enzyme Bos taurus
frozen enzyme is stable for at least 6 months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + N-acetylneuraminate
-
Equus caballus CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate
-
?
acetyl-CoA + N-acetylneuraminate the membrane-bound enzyme acts on endogenous, glycoprotein-bound N-acetyl- and N-glycolylneuraminic acids, the soluble enzyme acts on exogenous non-glycosidically bound N-acetyl- and N-glycolylneuraminic acids Bos taurus CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate
-
?
acetyl-CoA + N-glycolylneuraminate
-
Bos taurus ?
-
?
acetyl-CoA + sialic acid
-
Bos taurus CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate
-
?
acetyl-CoA + sialic acid
-
Equus caballus CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Bos taurus
37
-
-
Equus caballus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.56
-
CoA
-
Bos taurus