Literature summary for 2.3.1.45 extracted from

Schauer, R.
Biosynthesis of N-acetyl-O-acetylneuraminic acids. I. Incorporation of (14C) acetate into sections of the submaxillary salivary gland of ox and horse (1970), Hoppe-Seyler's Z. Physiol. Chem., 351, 595-602.

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General Stability

General Stability	Organism
activity is stable to the first cycle of freezing, cannot be refrozen after thawing	Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
diethyl dicarbonate	-	Bos taurus
N-bromosuccinimide	-	Bos taurus
Triton X-100	above 0.02%	Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + sialic acid	Bos taurus	-	CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate	-	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	ox	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
submaxillary gland	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + N-acetylneuraminate	-	Bos taurus	CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate	-	?
acetyl-CoA + sialic acid	-	Bos taurus	CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate	-	?

Synonyms

Synonyms	Comment	Organism
More	in early days of investigation of sialate O-acetyltransferase it was not possible to discriminate clearly between 7- or 9-O-acetylated sialic acids enzymatically formed on a micro scale in order to denominate the enzyme correctly, there also exists the possibility of the existence of more than one O-acetyltransferases incorporating acetyl-groups into different positions of the sialic acid side chain, in contrast a hypothesis is presented for the requirement of only one transferase specific for the 7-OH group, after insertion of the O-acetyl group at O-7 the ester residue may migrate to O-9 afterwards a second O-acetyl group can be transferred by the same enzyme to O-7, which may migrate to O-8 and open the way for linking a third acetyl group to O-7	Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
22	-	50% loss of activity after 20 min at room temperature	Bos taurus
37	-	activity is very labile	Bos taurus

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Release 2023.1 (January 2023)