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Literature summary for 2.3.1.44 extracted from

  • Iwersen, M.; Dora, H.; Kohla, G.; Gasa, S.; Schauer, R.
    Solubilisation and properties of the sialate-4-O-acetyltransferase from guinea pig liver (2003), Biol. Chem., 384, 1035-1047.
    View publication on PubMed
Search for more literature for 2.3.1.44

Inhibitors

Inhibitors Comment Organism Structure
3',5'-ADP 80% inhibition at 1 mM Cavia porcellus
3'-dephospho-coenzyme A 50% inhibition at 0.022 mM Cavia porcellus
ATP 20% inhibition at 1 mM Cavia porcellus
coenzyme A 50% inhibition at 0.013 mM Cavia porcellus
desulfo-coenzyme A 50% inhibition at 0.013 mM Cavia porcellus
IDP 30% inhibition at 1 mM Cavia porcellus
IMP 16% inhibition at 1 mM Cavia porcellus
ITP 58% inhibition at 1 mM Cavia porcellus
NADPH 18% inhibition at 1 mM Cavia porcellus
p-chloromercuribenzoate 100% inhibition at 1 mM Cavia porcellus
pyridoxal 5'-phosphate 75% inhibition at 1 mM Cavia porcellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 ganglioside GD3
-
 Cavia porcellus
0.0036
-
 acetyl-CoA
-
 Cavia porcellus

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
analysis of solubilisation by detergent CHAPS. Need for an unknown low molecular mass and heat-stable cofactor Cavia porcellus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 rapid method for measuring enzyme activity using ganglioside GD3 Cavia porcellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + ganglioside 9-O-acetyl-GD3 110% of the acitivity with ganglioside GD3 Cavia porcellus CoA + ganglioside 4,9-O-diacetyl-GD3
-
 ?
acetyl-CoA + ganglioside GD1a 90% of the acitivity with ganglioside GD3 Cavia porcellus CoA + 4-O-acetylganglioside GD1a
-
 ?
acetyl-CoA + ganglioside GD2 48% of the acitivity with ganglioside GD3 Cavia porcellus CoA + 4-O-acetylated ganglioside GD2
-
 ?
acetyl-CoA + ganglioside GD3
-
 Cavia porcellus CoA + 4-O-acetylated ganglioside GD3
-
 ?
acetyl-CoA + ganglioside GQ1b 125% of the acitivity with ganglioside GD3 Cavia porcellus CoA + 4-O-acetylated ganglioside GQ1b
-
 ?
acetyl-CoA + ganglioside GT1b 80% of the acitivity with ganglioside GD3 Cavia porcellus CoA + 4-O-acetylated ganglioside GT1b
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 solubilized enzyme, presence of KCl and K2HPO4 Cavia porcellus

pH Range

pH Minimum pH Maximum Comment Organism
5.6
-
 solubilized enzyme, presence of KCl and K2HPO4 Cavia porcellus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0148
-
 coenzyme A
-
 Cavia porcellus