Literature summary for 2.3.1.43 extracted from

Romanow, W.G.; Piper, D.E.; Fordstrom, P.; Thibault, S.; Zhou, M.; Walker, N.P.
BacMam production of active recombinant lecithin-cholesterol acyltransferase: expression, purification and characterization (2016), Protein Expr. Purif., 105, 1-6.

Application

Application	Comment	Organism
drug development	a therapeutic that increases enzyme LCAT activity may promote reverse cholesterol transport and prove beneficial for the treatment of dyslipidaemia and atherosclerosis	Homo sapiens
medicine	a therapeutic that increases enzyme LCAT activity may promote reverse cholesterol transport and prove beneficial for the treatment of dyslipidaemia and atherosclerosis	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
gene lcat, use of baculovirus as a transducing vector to express the C-or N-terminally His-tagged enzyme in insect cell line Sf9, and to transiently express tagged LCAT in human HEK-293 GnTI cells, deficient in N-acetylglucosaminyl transferase I and expression of the recombinant protein as a high-mannose glycoform (of the MAN9GlcNAc2 or MAN5GlcNAc2 types) suitable for deglycosylation by Endo H, which leaves a single N-acetyl glucosamine (GlcNAc) residue at the glycosylation sites, to avoid protein aggregation coccuring with fully deglycosylated protein. Or expression in HEK-293 6E cells. TEV or Factor Xa sites are introduced for tag cleavage. The enzyme is secreted. Method development and evaluation, overview	Homo sapiens

Cloned (Comment)

Organism

gene lcat, use of baculovirus as a transducing vector to express the C-or N-terminally His-tagged enzyme in insect cell line Sf9, and to transiently express tagged LCAT in human HEK-293 GnTI cells, deficient in N-acetylglucosaminyl transferase I and expression of the recombinant protein as a high-mannose glycoform (of the MAN9GlcNAc2 or MAN5GlcNAc2 types) suitable for deglycosylation by Endo H, which leaves a single N-acetyl glucosamine (GlcNAc) residue at the glycosylation sites, to avoid protein aggregation coccuring with fully deglycosylated protein. Or expression in HEK-293 6E cells. TEV or Factor Xa sites are introduced for tag cleavage. The enzyme is secreted. Method development and evaluation, overview

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
C31Y	site-directed mutagenesis, the mutation renders the enzyme more stable and active than the native form	Homo sapiens
additional information	removal of a proline rich region (residues 402 to 416) on the C-terminus	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	the enzyme is secreted	Homo sapiens	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
49000	62000	recombinant enzyme, gel filtration	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
phosphatidylcholine + a sterol	Homo sapiens	-	1-acylglycerophosphocholine + a sterol ester	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P04180	gene lcat	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	the enzyme is highly glycosylated with four potential N-linked glycosylation sites and two putative O-linked sites	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
recombinant partially deglycosylated His-tagged enzyme from HEK-293 GnTI cells by nickel affinity chromatography to homogeneity	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
blood plasma	-	Homo sapiens	-
liver	produced predominantly in the liver and secreted as a circulating protein into the serum	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphatidylcholine + a sterol	-	Homo sapiens	1-acylglycerophosphocholine + a sterol ester	-	?

Synonyms

Synonyms	Comment	Organism
LCAT	-	Homo sapiens
lecithin-cholesterol acyltransferase	-	Homo sapiens

General Information

General Information	Comment	Organism
evolution	LCAT is a member of the alpha/beta hydrolase family	Homo sapiens
physiological function	a key enzyme in the esterification of cholesterol and its subsequent incorporation into the core of high density lipoprotein (HDL) particles. The enzyme is also also involved in reverse cholesterol transport, the mechanism by which cholesterol is removed from peripheral cells and transported to the liver for excretion	Homo sapiens