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Literature summary for 2.3.1.43 extracted from

  • Albers, J.J.; Chen, C.H.; Lacko, A.G.
    Isolation, characterization, and assay of lecithin-cholesterol acyltransferase (1986), Methods Enzymol., 129, 763-783.
    View publication on PubMed
Search for more literature for 2.3.1.43

Activating Compound

Activating Compound Comment Organism Structure
apolipoprotein A-I absolute requirement with phosphatidylcholine-cholesterol vesicles as a substrate Homo sapiens
additional information apo-A-II, apo-C-III and apo-D inhibit activation of enzyme by apo-A-1 Homo sapiens

General Stability

General Stability Organism
inactivation at the air-water interface is prevented by buffered media of low ionic strength Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid)
-
 Homo sapiens
carnitine
-
 Homo sapiens
chlorpromazine
-
 Homo sapiens
cysteine
-
 Homo sapiens
diisopropyl fluorophosphate
-
 Homo sapiens
p-hydroxymercuribenzoate
-
 Homo sapiens
reduced glutathione
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, SDS-PAGE Homo sapiens
67000
-
 size exclusion chromatography Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylcholine + sterol Homo sapiens enzyme is believed to act on the surface of high-density lipoproteins, catalyzes the hydrolysis of fatty acids of the sn-2-position of phosphatidylcholine and transfers the fatty acid to free cholesterol to form cholesteryl ester, transesterification is the major source of plasma cholesteryl ester in human, enzyme has the key role in the transport of cholesterol from peripheral tissues to the liver, in the interconversion of HDL subclasses and the maintenance of lipoprotein structure 1-acylglycerophosphocholine + sterol ester
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 25% carbohydrate Homo sapiens
glycoprotein with a 5% sialic acid content Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
plasma
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.54
-
 at 37°C and pH: 7.4 Homo sapiens

Storage Stability

Storage Stability Organism
4°C, 0.4 mM phosphate buffer, 4 mM 2-mercaptoethanol, under N2, 4 weeks Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information lecithin-cholesterol single bilayer vesicles as substrate Homo sapiens ?
-
 ?
phosphatidylcholine + sterol cholesterol as substrate Homo sapiens 1-acylglycerophosphocholine + sterol ester
-
 r
phosphatidylcholine + sterol enzyme is believed to act on the surface of high-density lipoproteins, catalyzes the hydrolysis of fatty acids of the sn-2-position of phosphatidylcholine and transfers the fatty acid to free cholesterol to form cholesteryl ester, transesterification is the major source of plasma cholesteryl ester in human, enzyme has the key role in the transport of cholesterol from peripheral tissues to the liver, in the interconversion of HDL subclasses and the maintenance of lipoprotein structure Homo sapiens 1-acylglycerophosphocholine + sterol ester
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 transesterification of high-density lipoproteins and liposomes Homo sapiens

Cofactor

Cofactor Comment Organism Structure
apolipoprotein A1
-
 Homo sapiens