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Literature summary for 2.3.1.43 extracted from

  • Doi, Y.; Nishida, T.
    Lecithin-cholesterol acyltransferase from human plasma (1981), Methods Enzymol., 71, 753-767.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
apolipoprotein A-I absolute requirement with phosphatidylcholine-cholesterol vesicles as a substrate Homo sapiens
Apolipoprotein C-1 cofactor activity, less effective than apo-A-1 Homo sapiens
additional information apo-A-II, apo-C-III and apo-D inhibit activation of enzyme by apo-A-1 Homo sapiens

General Stability

General Stability Organism
apo-A-1, albumin and lecithin-cholesterol vesicles stabilize Homo sapiens
glycerol stabilizes during storage at -20°C Homo sapiens
lability to freezing and thawing and to high ionic strength Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol at high concentration Homo sapiens
dithiothreitol at high concentration Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
sedimentation equilibrium ultracentrifugation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylcholine + sterol Homo sapiens important in metabolism and structure of plasma lipoproteins 1-acylglycerophosphocholine + sterol ester
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 24% carbohydrate by weight, 13 mol of mannose, 30 mol of galactose, 17 mol of glucosamine and 13 mol of sialic acid per mol of enzyme protein Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
plasma
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7
-
in cholesterol-lecithin vesicles Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information long-chain primary alcohols can act as acyl acceptors Homo sapiens ?
-
?
additional information lecithin-cholesterol single bilayer vesicles as substrate Homo sapiens ?
-
?
phosphatidylcholine + sterol wide specificity for acyl acceptor: sterols with a beta-configuration at carbon-3 and trans fused A /B rings Homo sapiens 1-acylglycerophosphocholine + sterol ester
-
r
phosphatidylcholine + sterol cholesterol as substrate Homo sapiens 1-acylglycerophosphocholine + sterol ester
-
r
phosphatidylcholine + sterol acyl donor: high degree of specificity for phospholipids containing a basic nitrogen atom Homo sapiens 1-acylglycerophosphocholine + sterol ester
-
r
phosphatidylcholine + sterol important in metabolism and structure of plasma lipoproteins Homo sapiens 1-acylglycerophosphocholine + sterol ester
-
?