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Literature summary for 2.3.1.42 extracted from
- Dihydroxyacetone phosphate acyltransferase (1997), Biochim. Biophys. Acta, 1348, 27-34.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetaldehyde | - |
Cavia porcellus |  |
| Isopropanol | stimulation | Cavia porcellus | |
| additional information | heat shock, osmotic shock and freeze-thaw cycles increase enzyme activity | Cavia porcellus | |
| Phospholipid | stimulates partially purified enzyme, not membrane-bound | Cavia porcellus | |
General Stability
| General Stability | Organism |
|---|---|
| heat shock, osmotic shock and freeze-thaw cycles increase enzyme activity at pH 7.5, at pH 5.5 activity is decreased | Cavia porcellus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithio-bis(2-nitrobenzoate) | i.e. DTNB; no inhibition | Cavia porcellus | |
| 5,5'-dithio-bis(2-nitrobenzoate) | i.e. DTNB; no inhibition | Homo sapiens | |
| 5,5'-dithio-bis(2-nitrobenzoate) | i.e. DTNB; no inhibition | Oryctolagus cuniculus | |
| 5,5'-dithio-bis(2-nitrobenzoate) | i.e. DTNB; no inhibition | Rattus norvegicus | |
| 5,5'-dithio-bis(2-nitrobenzoate) | i.e. DTNB; no inhibition | Saccharomyces cerevisiae | |
| Hg2+ | - |
Cavia porcellus | |
| N-ethylmaleimide | no inhibition | Cavia porcellus | |
| N-ethylmaleimide | no inhibition | Homo sapiens | |
| N-ethylmaleimide | no inhibition | Oryctolagus cuniculus | |
| N-ethylmaleimide | no inhibition | Rattus norvegicus | |
| N-ethylmaleimide | no inhibition | Saccharomyces cerevisiae | |
| Pb2+ | - |
Cavia porcellus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Cavia porcellus | |
| additional information | - |
additional information | - |
Rattus norvegicus | |
| 0.04 | 0.06 | dihydroxyacetone phosphate | low concentration of substrate | Cavia porcellus | |
| 0.2 | 0.25 | dihydroxyacetone phosphate | high concentration of substrate | Cavia porcellus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Cavia porcellus | - |
- |
| microsome | - |
Homo sapiens | - |
- |
| microsome | - |
Rattus norvegicus | - |
- |
| microsome | - |
Oryctolagus cuniculus | - |
- |
| peroxisome | membrane-bound | Cavia porcellus | 5777 | - |
| peroxisome | membrane-bound | Homo sapiens | 5777 | - |
| peroxisome | membrane-bound | Rattus norvegicus | 5777 | - |
| peroxisome | membrane-bound | Oryctolagus cuniculus | 5777 | - |
| peroxisome | microperoxisome of brain, intestinal mucosa | Rattus norvegicus | 5777 | - |
| peroxisome | catalytic site at inner surface of peroxisomal membrane | Cavia porcellus | 5777 | - |
| peroxisome | catalytic site at inner surface of peroxisomal membrane | Homo sapiens | 5777 | - |
| peroxisome | catalytic site at inner surface of peroxisomal membrane | Rattus norvegicus | 5777 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | stimulation | Cavia porcellus | |
| additional information | - |
Oryctolagus cuniculus | |
| additional information | no activation by Mg2+, partially purified | Cavia porcellus | |
| additional information | no activation by Mg2+, partially purified | Rattus norvegicus | |
| additional information | no activation by Mn2+, partially purified | Cavia porcellus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | enzyme activity is reduced with disorders: neonatal adrenoleukodystrophy, infantile Refsum disease, and hyperpipecolic acidemia | ? | - |
? | |
| additional information | Homo sapiens | enzyme amount is reduced by 90% in Zellweger syndrome | ? | - |
? | |
| additional information | Homo sapiens | patients affected with rhizomelic chondrodysplasia punctata show reduced enzyme activity due to a defect in peroxisomal targeting | ? | - |
? | |
| palmitoyl-CoA + dihydroxyacetone phosphate | Saccharomyces cerevisiae | no precursor for glycerol ether lipids | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | Cavia porcellus | obligate precursor in ether lipids biosynthesis | CoA + monopalmitoyldihydroxyacetone phosphate | - |
r | |
| palmitoyl-CoA + dihydroxyacetone phosphate | Homo sapiens | obligate precursor in ether lipids biosynthesis | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | Rattus norvegicus | obligate precursor in ether lipids biosynthesis | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | Saccharomyces cerevisiae | obligate precursor in ether lipids biosynthesis | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | Oryctolagus cuniculus | obligate precursor in ether lipids biosynthesis | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | Cavia porcellus | reaction in phosphatidic acid biosynthesis | CoA + monopalmitoyldihydroxyacetone phosphate | - |
r | |
| palmitoyl-CoA + dihydroxyacetone phosphate | Homo sapiens | reaction in phosphatidic acid biosynthesis | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | Rattus norvegicus | reaction in phosphatidic acid biosynthesis | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | Saccharomyces cerevisiae | reaction in phosphatidic acid biosynthesis | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | Oryctolagus cuniculus | reaction in phosphatidic acid biosynthesis | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| no activity in Schizosaccharomyces pombe | - |
- |
- |
| Oryctolagus cuniculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces pastorianus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipocyte | - |
Cavia porcellus | - |
| adipocyte | - |
Rattus norvegicus | - |
| brain | - |
Rattus norvegicus | - |
| harderian gland | - |
Oryctolagus cuniculus | - |
| intestine | mucosa | Rattus norvegicus | - |
| kidney | - |
Cavia porcellus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Cavia porcellus | - |
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Oryctolagus cuniculus | - |
| placenta | - |
Homo sapiens | - |
| skin fibroblast | cell culture | Homo sapiens | - |
| spleen | - |
Cavia porcellus | - |
| spleen | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay methods, overview | Cavia porcellus |
| additional information | - |
assay methods, overview | Homo sapiens |
| additional information | - |
assay methods, overview | Rattus norvegicus |
| additional information | - |
assay methods, overview | Saccharomyces cerevisiae |
| additional information | - |
assay methods, overview | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + dihydroxyacetone phosphate | - |
Cavia porcellus | CoA + acyldihydroxyacetone phosphate | - |
r | |
| acyl-CoA + dihydroxyacetone phosphate | - |
Homo sapiens | CoA + acyldihydroxyacetone phosphate | - |
ir | |
| acyl-CoA + dihydroxyacetone phosphate | - |
Rattus norvegicus | CoA + acyldihydroxyacetone phosphate | - |
ir | |
| acyl-CoA + dihydroxyacetone phosphate | - |
Saccharomyces cerevisiae | CoA + acyldihydroxyacetone phosphate | - |
ir | |
| acyl-CoA + dihydroxyacetone phosphate | - |
Oryctolagus cuniculus | CoA + acyldihydroxyacetone phosphate | - |
ir | |
| additional information | enzyme activity is reduced with disorders: neonatal adrenoleukodystrophy, infantile Refsum disease, and hyperpipecolic acidemia | Homo sapiens | ? | - |
? | |
| additional information | enzyme amount is reduced by 90% in Zellweger syndrome | Homo sapiens | ? | - |
? | |
| additional information | patients affected with rhizomelic chondrodysplasia punctata show reduced enzyme activity due to a defect in peroxisomal targeting | Homo sapiens | ? | - |
? | |
| palmitoyl-CoA + dihydroxyacetone phosphate | - |
Cavia porcellus | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | - |
Homo sapiens | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | - |
Rattus norvegicus | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | - |
Saccharomyces cerevisiae | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | - |
Oryctolagus cuniculus | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | no precursor for glycerol ether lipids | Saccharomyces cerevisiae | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | obligate precursor in ether lipids biosynthesis | Cavia porcellus | CoA + monopalmitoyldihydroxyacetone phosphate | - |
r | |
| palmitoyl-CoA + dihydroxyacetone phosphate | obligate precursor in ether lipids biosynthesis | Homo sapiens | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | obligate precursor in ether lipids biosynthesis | Rattus norvegicus | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | obligate precursor in ether lipids biosynthesis | Saccharomyces cerevisiae | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | obligate precursor in ether lipids biosynthesis | Oryctolagus cuniculus | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | reaction in phosphatidic acid biosynthesis | Cavia porcellus | CoA + monopalmitoyldihydroxyacetone phosphate | - |
r | |
| palmitoyl-CoA + dihydroxyacetone phosphate | reaction in phosphatidic acid biosynthesis | Homo sapiens | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | reaction in phosphatidic acid biosynthesis | Rattus norvegicus | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | reaction in phosphatidic acid biosynthesis | Saccharomyces cerevisiae | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| palmitoyl-CoA + dihydroxyacetone phosphate | reaction in phosphatidic acid biosynthesis | Oryctolagus cuniculus | CoA + monopalmitoyldihydroxyacetone phosphate | - |
ir | |
| stearoyl-CoA + dihydroxyacetone phosphate | - |
Cavia porcellus | CoA + stearoyldihydroxyacetone phosphate | - |
ir | |
| stearoyl-CoA + dihydroxyacetone phosphate | - |
Homo sapiens | CoA + stearoyldihydroxyacetone phosphate | - |
ir | |
| stearoyl-CoA + dihydroxyacetone phosphate | - |
Rattus norvegicus | CoA + stearoyldihydroxyacetone phosphate | - |
ir | |
| stearoyl-CoA + dihydroxyacetone phosphate | - |
Saccharomyces cerevisiae | CoA + stearoyldihydroxyacetone phosphate | - |
ir | |
| stearoyl-CoA + dihydroxyacetone phosphate | - |
Oryctolagus cuniculus | CoA + stearoyldihydroxyacetone phosphate | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DHAPAT | - |
Cavia porcellus |
| DHAPAT | - |
Homo sapiens |
| DHAPAT | - |
Rattus norvegicus |
| DHAPAT | - |
Saccharomyces cerevisiae |
| DHAPAT | - |
Oryctolagus cuniculus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
membrane-bound: heat-stable | Cavia porcellus |
| additional information | - |
membrane-bound: heat-stable | Rattus norvegicus |
| 55 | - |
- |
Rattus norvegicus |
| 55 | - |
15 min stable, crude peroxisomal preparation | Cavia porcellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
overview | Cavia porcellus |
| additional information | - |
overview | Homo sapiens |
| additional information | - |
overview | Rattus norvegicus |
| additional information | - |
overview | Saccharomyces cerevisiae |
| additional information | - |
overview | Oryctolagus cuniculus |
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