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Literature summary for 2.3.1.42 extracted from
- Rat liver dihydroxyacetone-phosphate acyltransferases and their contribution to glycerolipid synthesis (1984), J. Biol. Chem., 259, 9064-9075.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | peroxisomal enzyme can be used as marker enzyme for peroxisomal membrane due to insensitivity to glycerol-3-phosphate | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| deoxycholate | inactivation | Rattus norvegicus |  |
| glycerol 3-phosphate | strong, extraperoxisomal activities | Rattus norvegicus | |
| N-ethylmaleimide | mitochondrial enzyme: weak; not peroxisomal enzyme | Rattus norvegicus | |
| SDS | - |
Rattus norvegicus | |
| Triton X-100 | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Rattus norvegicus | 5783 | - |
| microsome | - |
Rattus norvegicus | - |
- |
| mitochondrion | membrane-bound, may be side-reaction of glycerol 3-phosphate acyltransferase | Rattus norvegicus | 5739 | - |
| additional information | suborganellar localization | Rattus norvegicus | - |
- |
| peroxisome | predominantly, marker enzyme for peroxisomal membrane | Rattus norvegicus | 5777 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| palmitoyl-CoA + dihydroxyacetone phosphate | Rattus norvegicus | peroxisomal enzyme involved in ether lipid biosynthesis | CoA + monopalmitoyldihydroxyacetone phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
female Wistar | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + dihydroxyacetone phosphate | - |
Rattus norvegicus | CoA + acyldihydroxyacetone phosphate | i.e. acylglycerone phosphate | ? | |
| palmitoyl-CoA + dihydroxyacetone phosphate | mitochondrial enzyme | Rattus norvegicus | CoA + monopalmitoyldihydroxyacetone phosphate | - |
? | |
| palmitoyl-CoA + dihydroxyacetone phosphate | peroxisomal enzyme involved in ether lipid biosynthesis | Rattus norvegicus | CoA + monopalmitoyldihydroxyacetone phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | 6 | intact peroxisomes | Rattus norvegicus |
| 7 | 8 | mitochondrial enzyme | Rattus norvegicus |
| 7 | - |
purified microsomes | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.1 | 8.5 | about half-maximal activity at pH 5.1 and pH 8.5 | Rattus norvegicus |
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Release 2023.1 (January 2023)
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