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Literature summary for 2.3.1.42 extracted from
- Dihydroxyacetone phosphate acyltransferase (1992), Methods Enzymol., 209, 92-98.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| asolectin | activation, i.e. crude soybean phospholipid preparation containing phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol | Cavia porcellus | |
| bovine serum albumin | activation, by inhibiting the formation of palmitoyl-CoA micelles | Cavia porcellus | |
| additional information | heating membranes to 50°C prior to assay increases activity of membrane-bound enzyme | Cavia porcellus | |
| Phospholipid | - |
Cavia porcellus |  |
General Stability
| General Stability | Organism |
|---|---|
| heating membranes to 50°C prior to assay increases activity of membrane-bound enzyme | Cavia porcellus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithio-bis(2-nitrobenzoate) | 5 mM, moderate; i.e. DTNB | Cavia porcellus | |
| iodoacetamide | not | Cavia porcellus | |
| N-ethylmaleimide | not peroxisomal enzyme | Cavia porcellus | |
| p-chloromercuriphenylsulfonic acid | moderate | Cavia porcellus | |
| palmitoyl-CoA | bovine serum albumin protects | Cavia porcellus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Cavia porcellus | |
| 0.04 | 0.2 | dihydroxyacetone phosphate | membrane-bound enzyme, pH 7.4 | Cavia porcellus | |
| 0.06 | - |
dihydroxyacetone phosphate | purified enzyme, pH 7.4 | Cavia porcellus | |
| 0.1 | 0.5 | dihydroxyacetone phosphate | solubilized, pH 7.4 | Cavia porcellus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| peroxisome | - |
Cavia porcellus | 5777 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69000 | - |
x * 69000, SDS-PAGE | Cavia porcellus |
| 90000 | - |
gel filtration | Cavia porcellus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.35 | - |
purified enzyme | Cavia porcellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + dihydroxyacetone phosphate | - |
Cavia porcellus | CoA + acyldihydroxyacetone phosphate | i.e. acylglycerone phosphate | ? | |
| palmitoyl-CoA + dihydroxyacetone phosphate | - |
Cavia porcellus | CoA + monopalmitoyldihydroxyacetone phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 69000, SDS-PAGE | Cavia porcellus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Cavia porcellus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
membrane-bound: heat-stable | Cavia porcellus |
| additional information | - |
solubilized: heat-labile | Cavia porcellus |
| 40 | - |
significant loss of activity | Cavia porcellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
membrane-bound peroxisomal enzyme | Cavia porcellus |
| 7.4 | - |
detergent-solubilized enzyme | Cavia porcellus |
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