Activating Compound | Comment | Organism | Structure |
---|---|---|---|
asolectin | activation, i.e. crude soybean phospholipid preparation containing phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol | Cavia porcellus | |
bovine serum albumin | activation, by inhibiting the formation of palmitoyl-CoA micelles | Cavia porcellus | |
additional information | heating membranes to 50°C prior to assay increases activity of membrane-bound enzyme | Cavia porcellus | |
Phospholipid | - |
Cavia porcellus |
General Stability | Organism |
---|---|
heating membranes to 50°C prior to assay increases activity of membrane-bound enzyme | Cavia porcellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithio-bis(2-nitrobenzoate) | 5 mM, moderate; i.e. DTNB | Cavia porcellus | |
iodoacetamide | not | Cavia porcellus | |
N-ethylmaleimide | not peroxisomal enzyme | Cavia porcellus | |
p-chloromercuriphenylsulfonic acid | moderate | Cavia porcellus | |
palmitoyl-CoA | bovine serum albumin protects | Cavia porcellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Cavia porcellus | |
0.04 | 0.2 | dihydroxyacetone phosphate | membrane-bound enzyme, pH 7.4 | Cavia porcellus | |
0.06 | - |
dihydroxyacetone phosphate | purified enzyme, pH 7.4 | Cavia porcellus | |
0.1 | 0.5 | dihydroxyacetone phosphate | solubilized, pH 7.4 | Cavia porcellus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Cavia porcellus | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
x * 69000, SDS-PAGE | Cavia porcellus |
90000 | - |
gel filtration | Cavia porcellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Cavia porcellus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.35 | - |
purified enzyme | Cavia porcellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + dihydroxyacetone phosphate | - |
Cavia porcellus | CoA + acyldihydroxyacetone phosphate | i.e. acylglycerone phosphate | ? | |
palmitoyl-CoA + dihydroxyacetone phosphate | - |
Cavia porcellus | CoA + monopalmitoyldihydroxyacetone phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 69000, SDS-PAGE | Cavia porcellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Cavia porcellus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
membrane-bound: heat-stable | Cavia porcellus |
additional information | - |
solubilized: heat-labile | Cavia porcellus |
40 | - |
significant loss of activity | Cavia porcellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
membrane-bound peroxisomal enzyme | Cavia porcellus |
7.4 | - |
detergent-solubilized enzyme | Cavia porcellus |