Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.41 extracted from

  • He, X.; Reynolds, K.A.
    Purification, characterization, and identification of novel inhibitors of the beta-ketoacyl-acyl carrier protein synthase III (FabH) from Staphylococcus aureus (2002), Antimicrob. Agents Chemother., 46, 1310-1318.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
pharmacology synthase III is a target for drug development against multi-drug resistant strains Staphylococcus aureus

Cloned(Commentary)

Cloned (Comment) Organism
synthase III, overexpression of His-tagged FabH in Escherichia coli BL21 (DE3) Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
4-phenyl-5-phenylimino-[1,2,4]dithiazolidin-3-one i.e. HR19; strong, synthase III Staphylococcus aureus
5-chloro-4-phenyl-[1,2]-dithiol-3-one complete inhibition at 0.01 nM, synthase III; i.e. HR12 Staphylococcus aureus
cerulenin weak, synthase III Staphylococcus aureus
thiolactomycin synthase III; weak Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00032
-
isobutyryl-CoA synthase III Staphylococcus aureus
0.00232
-
butyryl-CoA synthase III Staphylococcus aureus
0.00618
-
acetyl-CoA synthase III Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
amino acid sequence alignment Staphylococcus aureus
37000
-
2 * 37000, recombinant synthase III, SDS-PAGE Staphylococcus aureus
67600
-
gel filtration Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] Staphylococcus aureus initiation of fatty acid biosynthesis 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
ir
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] Staphylococcus aureus part of fatty acid synthase II 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
ir

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus P99159 synthase III, gene fabH
-
Staphylococcus aureus P99159 strain ATCC 35556
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged synthase III from E. coli Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + malonyl-[acyl-carrier protein]
-
Staphylococcus aureus ?
-
?
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] synthase III Staphylococcus aureus acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
ir
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] initiation of fatty acid biosynthesis Staphylococcus aureus 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
ir
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] part of fatty acid synthase II Staphylococcus aureus 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
ir
butyryl-CoA + malonyl-[acyl-carrier protein]
-
Staphylococcus aureus ?
-
?
butyryl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] synthase III Staphylococcus aureus beta-ketohexanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
isobutyryl-CoA + malonyl-[acyl-carrier protein] synthase III Staphylococcus aureus ?
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
Staphylococcus aureus 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
ir

Subunits

Subunits Comment Organism
dimer 2 * 37000, recombinant synthase III, SDS-PAGE Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
synthase III Staphylococcus aureus

pH Range

pH Minimum pH Maximum Comment Organism
5.8 8 synthase III Staphylococcus aureus