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Literature summary for 2.3.1.40 extracted from

  • Taylor, S.S.; Heath, E.C.
    The incorporation of beta-hydroxy fatty acids into a phospholipid of Escherichia coli B (1969), J. Biol. Chem., 244, 6605-6616.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no stimulation by coenzyme A, ATP, GTP, UTP, glucosamine 1-phosphate, UDP-N-acetylglucosamine, Triton X-100, lipid A, alkaline treated lipid A or alkaline treated lipid A annealed with Escherichia coli phospholipids Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
particle-bound
-
Escherichia coli
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activation, 0.2 M Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
B
-
Escherichia coli B / ATCC 11303
-
B
-

Purification (Commentary)

Purification (Comment) Organism
partial, solubilization by sonic disruption Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-(acyl-carrier protein) + O-(2-acyl-sn-glycero-3-phospho) ethanolamine specific for C1-position of glycerol-phosphate backbone, the acyl-component of the acyl-(acyl-carrier-protein) may be 3-hydroxydecanoate, 3-hydroxylaurate, 3-hydroxymyristate and 3-hydroxyoctanoate (tentatively identified) Escherichia coli (acyl-carrier protein) + O-(1,2-diacyl-sn-glycero-3-phospho) ethanolamine
-
?
acyl-(acyl-carrier protein) + O-(2-acyl-sn-glycero-3-phospho) ethanolamine specific for C1-position of glycerol-phosphate backbone, the acyl-component of the acyl-(acyl-carrier-protein) may be 3-hydroxydecanoate, 3-hydroxylaurate, 3-hydroxymyristate and 3-hydroxyoctanoate (tentatively identified) Escherichia coli B / ATCC 11303 (acyl-carrier protein) + O-(1,2-diacyl-sn-glycero-3-phospho) ethanolamine
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
inactivation after 2-5 min Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
5 10 about half-maximal activity at pH 5.0, about 60% of maximal activity at pH 10.0 Escherichia coli