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Literature summary for 2.3.1.39 extracted from
- Alteration of the substrate specificity of the malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase domain of the multifunctional fatty acid synthase by mutation of a single arginine residue (1997), J. Biol. Chem., 272, 11975-11978.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of mutant proteins R606A and R606K in Escherichia coli | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0013 | - |
malonyl-CoA | R606K mutant | Rattus norvegicus |  |
| 0.0019 | - |
malonyl-CoA | wild-type | Rattus norvegicus | |
| 0.0162 | - |
malonyl-CoA | R606A mutant | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
mutant proteins R606A and R606K, Western analysis | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
malonyl transferase domain of EC 2.3.1.85 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of the mutants R606A und R606K | Rattus norvegicus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| of the mutant proteins R606A and R606K | Rattus norvegicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.015 | - |
R606A mutant | Rattus norvegicus |
| 0.154 | - |
R606K mutant | Rattus norvegicus |
| 1.61 | - |
wild-type | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| malonyl-CoA + pantetheine | reaction catalysed by the malonyl transferase domain of EC 2.3.1.85 | Rattus norvegicus | malonyl-pantetheine + CoA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | in vertebrates, yeast and mycobacteria malonyl transferase activity is a domain of the multifunctional polypeptide chains of fatty acid synthase EC 2.3.1.85 and EC 2.3.1.86 | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
malonyl CoA | R606A mutant | Rattus norvegicus | |
| 0.12 | - |
malonyl CoA | R606K mutant | Rattus norvegicus | |
| 1.2 | - |
malonyl CoA | wild-type | Rattus norvegicus | |
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Release 2023.1 (January 2023)
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