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Literature summary for 2.3.1.37 extracted from

  • Lendrihas, T.; Zhang, J.; Hunter, G.A.; Ferreira, G.C.
    Arg-85 and Thr-430 in murine 5-aminolevulinate synthase coordinate acyl-CoA-binding and contribute to substrate specificity (2009), Protein Sci., 18, 1847-1859.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.37

Protein Variants

Protein Variants Comment Organism
R85K catalytic efficiency similar to wild-type Mus musculus
R85L 68fold increase in catalytic efficincy with substrate octanoyl-CoA Mus musculus
R85L/T430V strong decrease in catalytic efficiency Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00054
-
 butanoyl-CoA mutant R85L/T430V, pH 7.5, 30°C Mus musculus
0.0018
-
 octanoyl-CoA mutant R85L, pH 7.5, 30°C Mus musculus
0.0027
-
 butanoyl-CoA mutant R85K, pH 7.5, 30°C Mus musculus
0.0029
-
 succinyl-CoA wild-type, pH 7.5, 30°C Mus musculus
0.0034
-
 octanoyl-CoA wild-type, pH 7.5, 30°C Mus musculus
0.0055
-
 2-hydroxybutanoyl-CoA mutant R85K, pH 7.5, 30°C Mus musculus
0.0061
-
 butanoyl-CoA wild-type, pH 7.5, 30°C Mus musculus
0.0061
-
 2-hydroxybutanoyl-CoA mutant R85L, pH 7.5, 30°C Mus musculus
0.0075
-
 glutaryl-CoA mutant R85K, pH 7.5, 30°C Mus musculus
0.0093
-
 butanoyl-CoA mutant R85L, pH 7.5, 30°C Mus musculus
0.0098
-
 2-hydroxybutanoyl-CoA wild-type, pH 7.5, 30°C Mus musculus
0.0103
-
 octanoyl-CoA mutant R85K, pH 7.5, 30°C Mus musculus
0.0124
-
 succinyl-CoA mutant R85K, pH 7.5, 30°C Mus musculus
0.017
-
 glutaryl-CoA wild-type, pH 7.5, 30°C Mus musculus
0.0171
-
 glycine wild-type, cosubstrate octanoyl-CoA, pH 7.5, 30°C Mus musculus
0.0172
-
 glycine mutant R85K, cosubstrate butanoyl-CoA, pH 7.5, 30°C Mus musculus
0.0172
-
 octanoyl-CoA mutant R85L/T430V, pH 7.5, 30°C Mus musculus
0.0182
-
 glycine mutant R85K, cosubstrate 2-hydroxybutanoyl-CoA, pH 7.5, 30°C Mus musculus
0.02
-
 glycine mutant R85K, cosubstrate succinyl-CoA, pH 7.5, 30°C Mus musculus
0.0203
-
 succinyl-CoA mutant R85L, pH 7.5, 30°C Mus musculus
0.0212
-
 glycine mutant R85K, cosubstrate glutaryl-CoA, pH 7.5, 30°C Mus musculus
0.0221
-
 glycine wild-type, cosubstrate 2-hydroxybutanoyl-CoA, pH 7.5, 30°C Mus musculus
0.0242
-
 glycine wild-type, cosubstrate succinyl-CoA, pH 7.5, 30°C Mus musculus
0.0252
-
 glycine mutant R85K, cosubstrate octanoyl-CoA, pH 7.5, 30°C Mus musculus
0.0284
-
 glycine wild-type, cosubstrate glutaryl-CoA, pH 7.5, 30°C Mus musculus
0.0293
-
 glycine wild-type, cosubstrate butanoyl-CoA, pH 7.5, 30°C Mus musculus
0.0301
-
 glutaryl-CoA mutant R85L, pH 7.5, 30°C Mus musculus
0.0552
-
 glycine mutant R85L, cosubstrate octanoyl-CoA, pH 7.5, 30°C Mus musculus
0.0591
-
 glycine mutant R85L, cosubstrate 2-hydroxybutanoyl-CoA, pH 7.5, 30°C Mus musculus
0.0631
-
 glycine mutant R85L, cosubstrate succinyl-CoA, pH 7.5, 30°C Mus musculus
0.0703
-
 glycine mutant R85L, cosubstrate butanoyl-CoA, pH 7.5, 30°C Mus musculus
0.0706
-
 glycine mutant R85L, cosubstrate glutaryl-CoA, pH 7.5, 30°C Mus musculus
0.0742
-
 glycine mutant cosubstrate octanoyl-CoA, R85L/T430V, pH 7.5, 30°C Mus musculus
0.0742
-
 2-hydroxybutanoyl-CoA mutant R85L/T430V, pH 7.5, 30°C Mus musculus
0.0882
-
 glycine mutant cosubstrate butanoyl-CoA, R85L/T430V, pH 7.5, 30°C Mus musculus
0.0922
-
 glycine mutant cosubstrate 2-hydroxybutanoyl-CoA, R85L/T430V, pH 7.5, 30°C Mus musculus
0.096
-
 succinyl-CoA mutant R85L/T430V, pH 7.5, 30°C Mus musculus
0.0984
-
 glycine mutant cosubstrate succinyl-CoA, R85L/T430V, pH 7.5, 30°C Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxybutanoyl-CoA + glycine
-
 Mus musculus ? + CoA + CO2
-
 ?
butanoyl-CoA + glycine
-
 Mus musculus ? + CoA + CO2
-
 ?
glutaryl-CoA + glycine
-
 Mus musculus ? + CoA + CO2
-
 ?
octanoyl-CoA + glycine
-
 Mus musculus ? + CoA + CO2
-
 ?
succinyl-CoA + glycine
-
 Mus musculus 5-aminolevulinate + CoA + CO2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00002
-
 octanoyl-CoA mutant R85L/T430V, pH 7.5, 30°C Mus musculus
0.001
-
 butanoyl-CoA mutant R85L/T430V, pH 7.5, 30°C Mus musculus
0.002
-
 succinyl-CoA mutant R85L/T430V, pH 7.5, 30°C Mus musculus
0.01
-
 2-hydroxybutanoyl-CoA mutant R85L, pH 7.5, 30°C Mus musculus
0.016
-
 succinyl-CoA mutant R85L, pH 7.5, 30°C Mus musculus
0.03
-
 octanoyl-CoA mutant R85L, pH 7.5, 30°C Mus musculus
0.0335
-
 butanoyl-CoA mutant R85L, pH 7.5, 30°C Mus musculus
0.037
-
 glutaryl-CoA mutant R85L, pH 7.5, 30°C Mus musculus
0.047
-
 2-hydroxybutanoyl-CoA mutant R85K, pH 7.5, 30°C Mus musculus
0.05
-
 glutaryl-CoA mutant R85K, pH 7.5, 30°C Mus musculus
0.067
-
 2-hydroxybutanoyl-CoA wild-type, pH 7.5, 30°C Mus musculus
0.105
-
 butanoyl-CoA wild-type, pH 7.5, 30°C Mus musculus
0.107
-
 succinyl-CoA mutant R85K, pH 7.5, 30°C Mus musculus
0.117
-
 glutaryl-CoA wild-type, pH 7.5, 30°C Mus musculus
0.167
-
 succinyl-CoA wild-type, pH 7.5, 30°C Mus musculus
0.17
-
 butanoyl-CoA mutant R85K, pH 7.5, 30°C Mus musculus
0.172
-
 octanoyl-CoA mutant R85K, pH 7.5, 30°C Mus musculus
0.34
-
 octanoyl-CoA wild-type, pH 7.5, 30°C Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00002
-
 glycine mutant cosubstrate succinyl-CoA, R85L/T430V, pH 7.5, 30°C Mus musculus
0.00017
-
 glycine mutant R85K, cosubstrate glutaryl-CoA, pH 7.5, 30°C Mus musculus
0.00018
-
 glycine mutant R85L, cosubstrate 2-hydroxybutanoyl-CoA, pH 7.5, 30°C Mus musculus
0.0002
-
 glycine mutant R85L, cosubstrate succinyl-CoA, pH 7.5, 30°C Mus musculus
0.0002
-
 succinyl-CoA mutant R85L/T430V, pH 7.5, 30°C Mus musculus
0.0005
-
 glycine mutant R85L, cosubstrate butanoyl-CoA, pH 7.5, 30°C Mus musculus
0.0005
-
 glycine mutant R85L, cosubstrate octanoyl-CoA, pH 7.5, 30°C Mus musculus
0.0005
-
 butanoyl-CoA mutant R85L/T430V, pH 7.5, 30°C Mus musculus
0.0007
-
 glutaryl-CoA mutant R85K, pH 7.5, 30°C Mus musculus
0.0008
-
 succinyl-CoA mutant R85L, pH 7.5, 30°C Mus musculus
0.0012
-
 glutaryl-CoA mutant R85L, pH 7.5, 30°C Mus musculus
0.0018
-
 2-hydroxybutanoyl-CoA mutant R85L, pH 7.5, 30°C Mus musculus
0.0023
-
 glycine mutant R85K, cosubstrate 2-hydroxybutanoyl-CoA, pH 7.5, 30°C Mus musculus
0.0028
-
 glycine wild-type, cosubstrate 2-hydroxybutanoyl-CoA, pH 7.5, 30°C Mus musculus
0.0035
-
 butanoyl-CoA mutant R85L, pH 7.5, 30°C Mus musculus
0.0043
-
 glycine wild-type, cosubstrate butanoyl-CoA, pH 7.5, 30°C Mus musculus
0.0048
-
 glycine wild-type, cosubstrate glutaryl-CoA, pH 7.5, 30°C Mus musculus
0.005
-
 glycine mutant R85K, cosubstrate succinyl-CoA, pH 7.5, 30°C Mus musculus
0.0067
-
 glycine wild-type, cosubstrate succinyl-CoA, pH 7.5, 30°C Mus musculus
0.0068
-
 glutaryl-CoA wild-type, pH 7.5, 30°C Mus musculus
0.007
-
 2-hydroxybutanoyl-CoA wild-type, pH 7.5, 30°C Mus musculus
0.0083
-
 glycine mutant R85K, cosubstrate butanoyl-CoA, pH 7.5, 30°C Mus musculus
0.0085
-
 2-hydroxybutanoyl-CoA mutant R85K, pH 7.5, 30°C Mus musculus
0.0087
-
 glycine mutant R85K, cosubstrate octanoyl-CoA, pH 7.5, 30°C Mus musculus
0.0089
-
 succinyl-CoA mutant R85K, pH 7.5, 30°C Mus musculus
0.02
-
 glycine mutant cosubstrate octanoyl-CoA, R85L/T430V, pH 7.5, 30°C Mus musculus
0.02
-
 glycine wild-type, cosubstrate octanoyl-CoA, pH 7.5, 30°C Mus musculus
0.02
-
 butanoyl-CoA wild-type, pH 7.5, 30°C Mus musculus
0.022
-
 2-hydroxybutanoyl-CoA mutant R85L/T430V, pH 7.5, 30°C Mus musculus
0.055
-
 octanoyl-CoA mutant R85L, pH 7.5, 30°C Mus musculus
0.062
-
 butanoyl-CoA mutant R85K, pH 7.5, 30°C Mus musculus
0.113
-
 octanoyl-CoA wild-type, pH 7.5, 30°C Mus musculus
0.117
-
 octanoyl-CoA mutant R85K, pH 7.5, 30°C Mus musculus
0.13
-
 glycine mutant R85L, cosubstrate glutaryl-CoA, pH 7.5, 30°C Mus musculus
0.55
-
 succinyl-CoA wild-type, pH 7.5, 30°C Mus musculus