Protein Variants | Comment | Organism |
---|---|---|
R85K | catalytic efficiency similar to wild-type | Mus musculus |
R85L | 68fold increase in catalytic efficincy with substrate octanoyl-CoA | Mus musculus |
R85L/T430V | strong decrease in catalytic efficiency | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00054 | - |
butanoyl-CoA | mutant R85L/T430V, pH 7.5, 30°C | Mus musculus | |
0.0018 | - |
octanoyl-CoA | mutant R85L, pH 7.5, 30°C | Mus musculus | |
0.0027 | - |
butanoyl-CoA | mutant R85K, pH 7.5, 30°C | Mus musculus | |
0.0029 | - |
succinyl-CoA | wild-type, pH 7.5, 30°C | Mus musculus | |
0.0034 | - |
octanoyl-CoA | wild-type, pH 7.5, 30°C | Mus musculus | |
0.0055 | - |
2-hydroxybutanoyl-CoA | mutant R85K, pH 7.5, 30°C | Mus musculus | |
0.0061 | - |
butanoyl-CoA | wild-type, pH 7.5, 30°C | Mus musculus | |
0.0061 | - |
2-hydroxybutanoyl-CoA | mutant R85L, pH 7.5, 30°C | Mus musculus | |
0.0075 | - |
glutaryl-CoA | mutant R85K, pH 7.5, 30°C | Mus musculus | |
0.0093 | - |
butanoyl-CoA | mutant R85L, pH 7.5, 30°C | Mus musculus | |
0.0098 | - |
2-hydroxybutanoyl-CoA | wild-type, pH 7.5, 30°C | Mus musculus | |
0.0103 | - |
octanoyl-CoA | mutant R85K, pH 7.5, 30°C | Mus musculus | |
0.0124 | - |
succinyl-CoA | mutant R85K, pH 7.5, 30°C | Mus musculus | |
0.017 | - |
glutaryl-CoA | wild-type, pH 7.5, 30°C | Mus musculus | |
0.0171 | - |
glycine | wild-type, cosubstrate octanoyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0172 | - |
glycine | mutant R85K, cosubstrate butanoyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0172 | - |
octanoyl-CoA | mutant R85L/T430V, pH 7.5, 30°C | Mus musculus | |
0.0182 | - |
glycine | mutant R85K, cosubstrate 2-hydroxybutanoyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.02 | - |
glycine | mutant R85K, cosubstrate succinyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0203 | - |
succinyl-CoA | mutant R85L, pH 7.5, 30°C | Mus musculus | |
0.0212 | - |
glycine | mutant R85K, cosubstrate glutaryl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0221 | - |
glycine | wild-type, cosubstrate 2-hydroxybutanoyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0242 | - |
glycine | wild-type, cosubstrate succinyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0252 | - |
glycine | mutant R85K, cosubstrate octanoyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0284 | - |
glycine | wild-type, cosubstrate glutaryl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0293 | - |
glycine | wild-type, cosubstrate butanoyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0301 | - |
glutaryl-CoA | mutant R85L, pH 7.5, 30°C | Mus musculus | |
0.0552 | - |
glycine | mutant R85L, cosubstrate octanoyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0591 | - |
glycine | mutant R85L, cosubstrate 2-hydroxybutanoyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0631 | - |
glycine | mutant R85L, cosubstrate succinyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0703 | - |
glycine | mutant R85L, cosubstrate butanoyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0706 | - |
glycine | mutant R85L, cosubstrate glutaryl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0742 | - |
glycine | mutant cosubstrate octanoyl-CoA, R85L/T430V, pH 7.5, 30°C | Mus musculus | |
0.0742 | - |
2-hydroxybutanoyl-CoA | mutant R85L/T430V, pH 7.5, 30°C | Mus musculus | |
0.0882 | - |
glycine | mutant cosubstrate butanoyl-CoA, R85L/T430V, pH 7.5, 30°C | Mus musculus | |
0.0922 | - |
glycine | mutant cosubstrate 2-hydroxybutanoyl-CoA, R85L/T430V, pH 7.5, 30°C | Mus musculus | |
0.096 | - |
succinyl-CoA | mutant R85L/T430V, pH 7.5, 30°C | Mus musculus | |
0.0984 | - |
glycine | mutant cosubstrate succinyl-CoA, R85L/T430V, pH 7.5, 30°C | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxybutanoyl-CoA + glycine | - |
Mus musculus | ? + CoA + CO2 | - |
? | |
butanoyl-CoA + glycine | - |
Mus musculus | ? + CoA + CO2 | - |
? | |
glutaryl-CoA + glycine | - |
Mus musculus | ? + CoA + CO2 | - |
? | |
octanoyl-CoA + glycine | - |
Mus musculus | ? + CoA + CO2 | - |
? | |
succinyl-CoA + glycine | - |
Mus musculus | 5-aminolevulinate + CoA + CO2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00002 | - |
octanoyl-CoA | mutant R85L/T430V, pH 7.5, 30°C | Mus musculus | |
0.001 | - |
butanoyl-CoA | mutant R85L/T430V, pH 7.5, 30°C | Mus musculus | |
0.002 | - |
succinyl-CoA | mutant R85L/T430V, pH 7.5, 30°C | Mus musculus | |
0.01 | - |
2-hydroxybutanoyl-CoA | mutant R85L, pH 7.5, 30°C | Mus musculus | |
0.016 | - |
succinyl-CoA | mutant R85L, pH 7.5, 30°C | Mus musculus | |
0.03 | - |
octanoyl-CoA | mutant R85L, pH 7.5, 30°C | Mus musculus | |
0.0335 | - |
butanoyl-CoA | mutant R85L, pH 7.5, 30°C | Mus musculus | |
0.037 | - |
glutaryl-CoA | mutant R85L, pH 7.5, 30°C | Mus musculus | |
0.047 | - |
2-hydroxybutanoyl-CoA | mutant R85K, pH 7.5, 30°C | Mus musculus | |
0.05 | - |
glutaryl-CoA | mutant R85K, pH 7.5, 30°C | Mus musculus | |
0.067 | - |
2-hydroxybutanoyl-CoA | wild-type, pH 7.5, 30°C | Mus musculus | |
0.105 | - |
butanoyl-CoA | wild-type, pH 7.5, 30°C | Mus musculus | |
0.107 | - |
succinyl-CoA | mutant R85K, pH 7.5, 30°C | Mus musculus | |
0.117 | - |
glutaryl-CoA | wild-type, pH 7.5, 30°C | Mus musculus | |
0.167 | - |
succinyl-CoA | wild-type, pH 7.5, 30°C | Mus musculus | |
0.17 | - |
butanoyl-CoA | mutant R85K, pH 7.5, 30°C | Mus musculus | |
0.172 | - |
octanoyl-CoA | mutant R85K, pH 7.5, 30°C | Mus musculus | |
0.34 | - |
octanoyl-CoA | wild-type, pH 7.5, 30°C | Mus musculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00002 | - |
glycine | mutant cosubstrate succinyl-CoA, R85L/T430V, pH 7.5, 30°C | Mus musculus | |
0.00017 | - |
glycine | mutant R85K, cosubstrate glutaryl-CoA, pH 7.5, 30°C | Mus musculus | |
0.00018 | - |
glycine | mutant R85L, cosubstrate 2-hydroxybutanoyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0002 | - |
glycine | mutant R85L, cosubstrate succinyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0002 | - |
succinyl-CoA | mutant R85L/T430V, pH 7.5, 30°C | Mus musculus | |
0.0005 | - |
glycine | mutant R85L, cosubstrate butanoyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0005 | - |
glycine | mutant R85L, cosubstrate octanoyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0005 | - |
butanoyl-CoA | mutant R85L/T430V, pH 7.5, 30°C | Mus musculus | |
0.0007 | - |
glutaryl-CoA | mutant R85K, pH 7.5, 30°C | Mus musculus | |
0.0008 | - |
succinyl-CoA | mutant R85L, pH 7.5, 30°C | Mus musculus | |
0.0012 | - |
glutaryl-CoA | mutant R85L, pH 7.5, 30°C | Mus musculus | |
0.0018 | - |
2-hydroxybutanoyl-CoA | mutant R85L, pH 7.5, 30°C | Mus musculus | |
0.0023 | - |
glycine | mutant R85K, cosubstrate 2-hydroxybutanoyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0028 | - |
glycine | wild-type, cosubstrate 2-hydroxybutanoyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0035 | - |
butanoyl-CoA | mutant R85L, pH 7.5, 30°C | Mus musculus | |
0.0043 | - |
glycine | wild-type, cosubstrate butanoyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0048 | - |
glycine | wild-type, cosubstrate glutaryl-CoA, pH 7.5, 30°C | Mus musculus | |
0.005 | - |
glycine | mutant R85K, cosubstrate succinyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0067 | - |
glycine | wild-type, cosubstrate succinyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0068 | - |
glutaryl-CoA | wild-type, pH 7.5, 30°C | Mus musculus | |
0.007 | - |
2-hydroxybutanoyl-CoA | wild-type, pH 7.5, 30°C | Mus musculus | |
0.0083 | - |
glycine | mutant R85K, cosubstrate butanoyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0085 | - |
2-hydroxybutanoyl-CoA | mutant R85K, pH 7.5, 30°C | Mus musculus | |
0.0087 | - |
glycine | mutant R85K, cosubstrate octanoyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.0089 | - |
succinyl-CoA | mutant R85K, pH 7.5, 30°C | Mus musculus | |
0.02 | - |
glycine | mutant cosubstrate octanoyl-CoA, R85L/T430V, pH 7.5, 30°C | Mus musculus | |
0.02 | - |
glycine | wild-type, cosubstrate octanoyl-CoA, pH 7.5, 30°C | Mus musculus | |
0.02 | - |
butanoyl-CoA | wild-type, pH 7.5, 30°C | Mus musculus | |
0.022 | - |
2-hydroxybutanoyl-CoA | mutant R85L/T430V, pH 7.5, 30°C | Mus musculus | |
0.055 | - |
octanoyl-CoA | mutant R85L, pH 7.5, 30°C | Mus musculus | |
0.062 | - |
butanoyl-CoA | mutant R85K, pH 7.5, 30°C | Mus musculus | |
0.113 | - |
octanoyl-CoA | wild-type, pH 7.5, 30°C | Mus musculus | |
0.117 | - |
octanoyl-CoA | mutant R85K, pH 7.5, 30°C | Mus musculus | |
0.13 | - |
glycine | mutant R85L, cosubstrate glutaryl-CoA, pH 7.5, 30°C | Mus musculus | |
0.55 | - |
succinyl-CoA | wild-type, pH 7.5, 30°C | Mus musculus |