Protein Variants | Comment | Organism |
---|---|---|
S254A | increase in Km value for succinyl-Coa and kcat value. Removal of the side chain hydroxyl group alters the microenvironment of the PLP cofactor and hinders succinyl-CoA binding | Mus musculus |
S254T | decrease in kcat value without altering Km value | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
succinyl-CoA | mutant S254T, 30°C, pH 7.5 | Mus musculus | |
0.0013 | - |
succinyl-CoA | wild-type, 30°C, pH 7.5 | Mus musculus | |
0.032 | - |
succinyl-CoA | mutant S254A, 30°C, pH 7.5 | Mus musculus | |
18 | - |
glycine | mutant S254A, 30°C, pH 7.5 | Mus musculus | |
25 | - |
glycine | wild-type, 30°C, pH 7.5 | Mus musculus | |
27 | - |
glycine | mutant S254T, 30°C, pH 7.5 | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + glycine | - |
Mus musculus | 5-aminolevulinate + CoA + CO2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
glycine | mutant S254T, 30°C, pH 7.5 | Mus musculus | |
0.14 | - |
glycine | wild-type, 30°C, pH 7.5 | Mus musculus | |
0.27 | - |
glycine | mutant S254A, 30°C, pH 7.5 | Mus musculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
glycine | mutant S254T, 30°C, pH 7.5 | Mus musculus | |
0.0056 | - |
glycine | wild-type, 30°C, pH 7.5 | Mus musculus | |
0.0084 | - |
succinyl-CoA | mutant S254A, 30°C, pH 7.5 | Mus musculus | |
0.015 | - |
glycine | mutant S254A, 30°C, pH 7.5 | Mus musculus | |
0.042 | - |
succinyl-CoA | mutant S254T, 30°C, pH 7.5 | Mus musculus | |
0.11 | - |
succinyl-CoA | wild-type, 30°C, pH 7.5 | Mus musculus |